Changes in nuclear DNA and RNA during epidermal keratinization
Changes in nuclear DNA and RNA during epidermal keratinization
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表皮角化过程中核DNA和RNA的变化
DOI：
10.1007/bf00223065
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发表时间：
1977
影响因子：
3.6
通讯作者：
W. Epstein
中科院分区：
文献类型：
--
作者：
Hiroyuki Suzuki;K. Fukuyama;W. Epstein
采用间接酶切法同时观察DNA和RNA，直接希夫-铊反应法单独观察DNA，研究了豚鼠耳皮肤表皮细胞细胞核内核酸的分布。两种方法均得到了相似的结果。随着表皮细胞的分化，细胞核内DNA和RNA的分布逐渐发生变化。下层细胞中的DNA基本上定位于与传统电子显微镜看到的电子不透明成分相同的区域。然而，通过细胞化学处理，我们发现DNA并不存在于表面颗粒细胞细胞核的所有电子不透明区域。RNA存在于所有层细胞的核仁中，但其密度在上层颗粒细胞中也较低。我们假设，作为分化过程的一部分，颗粒细胞细胞核中的核酸逐渐减少，空间充满了新合成的电子致密蛋白。
The distribution of nucleic acids within nuclei of epidermal cells in skin from guinea-pig ear was investigated using an indirect enzyme digestion technique to observe both DNA and RNA, and a direct Schiff-Thallium reaction technique, to observe DNA alone. Similar results were obtained by both methods.The distribution of DNA and RNA change gradually in nuclei as epidermal cells differentiate. DNA in cells of the lower strata is localized in essentially the same areas in which electron-opaque components are seen by conventional electron microscopy. With the cytochemical treatments, however, we found that DNA is not present in all electron-opaque areas of nuclei in superficial granular cells. RNA is present in the nucleoli of cells in all layers, but its density is also lower in the upper granular cells.We postulate that nucleic acids in nuclei of granular cells gradually decrease and that the space is filled with newly synthesized electron-dense protein, as part of the differentiation process.