MALE STERILITY1 is required for tapetal development and pollen wall biosynthesis
MALE STERILITY1 is required for tapetal development and pollen wall biosynthesis
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DOI：
10.1105/tpc.107.054981
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发表时间：
2007-11-01
期刊：
影响因子：
11.6
通讯作者：
Wilson, Zoe A.
中科院分区：
文献类型：
--
作者：
Yang, Caiyun;Vizcay-Barrena, Gema;Wilson, Zoe A.
拟南芥 MALE STERILITY1 (MS1) 基因对于能存活的花粉形成至关重要，并且与 PHD-finger 类转录因子具有同源性；然而，它在花粉发育中的作用尚未完全确定。我们发现 MS1 转录似乎是由野生型 MS1 转录物或蛋白质自动调节的。使用功能性绿色荧光蛋白 (GFP) 融合来分析 MS1 的时间和空间表达，我们证明 MS1: GFP 蛋白位于绒毡层内的核内，并在四孢子晚期和小孢子释放之间以发育调控的方式表达，然后可能通过泛素依赖性蛋白水解作用快速分解。 MS1 表达的缺失会导致绒毡层分泌和外壁结构的变化。微阵列分析表明，260 个基因（228 个下调和 32 个上调）在年轻的 ms1 芽中表达发生了改变。这些基因主要与花粉壁和外壳的形成有关；然而，许多转录因子和 Cys 蛋白酶也被确定为 MS1 的假定主要调控靶标。 MS1的异位表达改变了营养基因表达的转录调控，导致植物发育不良，分枝水平增加，花朵部分可育，成熟花粉壁材料明显增加。因此，MS1 在绒毡层中花粉壁和花粉外壳材料的诱导以及最终有活力的花粉的产生中发挥着关键作用。
The Arabidopsis thaliana MALE STERILITY1 (MS1) gene is critical for viable pollen formation and has homology to the PHD-finger class of transcription factors; however, its role in pollen development has not been fully defined. We show that MS1 transcription appears to be autoregulated by the wild-type MS1 transcript or protein. Using a functional green fluorescent protein (GFP) fusion to analyze the temporal and spatial expression of MS1, we demonstrate that the MS1: GFP protein is nuclear localized within the tapetum and is expressed in a developmentally regulated manner between late tetraspore and microspore release, then rapidly breaks down, probably by ubiquitin-dependent proteolysis. Absence of MS1 expression results in changes in tapetal secretion and exine structure. Microarray analysis has shown that 260 (228 downregulated and 32 upreglated) genes have altered expression in young ms1 buds. These genes are primarily associated with pollen wall and coat formation; however, a number of transcription factors and Cys proteases have also been identified as the putative primary regulatory targets of MS1. Ectopic expression of MS1 alters transcriptional regulation of vegetative gene expression, resulting in stunted plants with increased levels of branching, partially fertile flowers and an apparent increase in wall material on mature pollen. MS1 therefore plays a critical role in the induction of pollen wall and pollen coat materials in the tapetum and, ultimately, the production of viable pollen.