The somatostatin receptor subtype on rat enterochromaffinlike cells.
The somatostatin receptor subtype on rat enterochromaffinlike cells.
复制标题
大鼠肠嗜铬样细胞上的生长抑素受体亚型。
DOI：
10.1016/0016-5085(94)90231-3
复制
发表时间：
1994
期刊：
影响因子：
29.4
通讯作者：
Wu,SV
中科院分区：
文献类型：
--
作者：
Prinz,C;Sachs,G;Walsh,JH;Coy,DH;Wu,SV
背景/目的：胃肠道嗜铬样细胞（ECL）在外周调节胃酸分泌中起重要作用。方法：采用酶消化、淘洗、密度梯度离心和培养相结合的方法，从大鼠胃底黏膜分离ECL细胞，纯度为90%~ 95%。结果：以ECL细胞互补DNA文库为模板，用5种生长抑素受体亚型特异性引物进行聚合酶链反应，结果显示ECL细胞互补DNA中明显富集生长抑素2型受体。培养的高纯度ECL细胞的单细胞视频成像显示，只有生长抑素受体2型选择性激动剂DC 32-87在10− 11 mol/L时抑制胃泌素诱导的钙信号。3型和4型选择性激动剂DC 25-12和DC 32-92，以及生长抑素14需要100-1000倍的浓度（10− 8 mol/L）。生长抑素2型受体类似物也能抑制胃泌素刺激的组胺释放，其半数抑制浓度（IC 50）为2 × 10− 12 mol/L，而生长抑素14、3型和4型受体类似物的IC 50值为1 ~ 5 × 10− 9 mol/L。它们通过干扰胃泌素诱导的钙信号来抑制组胺分泌。
Background/Aims:Gastric enterochromaffinlike (ECL) cells play an important role in peripheral regulation of acid secretion. This study investigated the somatostatin receptor subtype on ECL cells.Methods:ECL cells were isolated from rat fundic mucosa to a purity of 90%–95% by combining enzymatic digestion, elutriation, density gradient centrifugation, and culture.Results:Polymerase chain reaction performed with templates from an ECL cell complementary DNA library and primers specific to each of the five known somatostatin receptor subtypes showed that the somatostatin receptor type 2 was significantly enriched in ECL complementary DNA. Single cell videoimaging of highly purified ECL cells in culture showed that only the somatostatin receptor type 2 selective agonist, DC 32–87, inhibited the gastrin-induced calcium signal at 10−11mol/L. The type 3 and type 4 selective agonists, DC 25-12 and DC 32–92, and also somatostatin 14 required 100–1000 times higher concentrations (10−8mol/L). The somatostatin receptor type 2 analogue also inhibited gastrin-stimulated histamine release with a 50% inhibitory concentration (IC50) value of 2 × 10−12mol/L, whereas somatostatin 14 and the type 3 and 4 analogues showed IC50values of 1 to 5 × 10−9mol/L.Conclusions:The predominant somatostatin receptors on rat gastric ECL cells are of the somatostatin receptor 2 subtype; they inhibit histamine secretion by interfering with the gastrin-induced calcium signal.