Enhanced Osseointegration Capability of Poly(ether ether ketone) via Combined Phosphate and Calcium Surface-Functionalization
Enhanced Osseointegration Capability of Poly(ether ether ketone) via Combined Phosphate and Calcium Surface-Functionalization
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DOI：
10.3390/ijms21010198
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发表时间：
2019-12
影响因子：
5.6
通讯作者：
Sunarso;A. Tsuchiya;R. Toita;K. Tsuru;K. Ishikawa
中科院分区：
文献类型：
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作者：
Sunarso;A. Tsuchiya;R. Toita;K. Tsuru;K. Ishikawa
聚醚醚酮（PEEK）的生物医学应用因其固有的生物惰性和缺乏骨整合能力而受到阻碍。在本研究中，为了增强PEEK的成骨活性，从而增强PEEK的骨整合能力，我们提出了一种结合磷酸盐和钙表面功能化的策略，其中臭氧气体处理和湿化学分别用于引入羟基以及磷酸盐和/或钙的改性。表面功能化显着提高了表面亲水性，而不改变表面粗糙度或形貌。细胞研究表明，与裸 PEEK 相比，磷酸盐或钙的固定增加了大鼠间充质干细胞的成骨作用，包括细胞增殖、碱性磷酸酶活性和骨样结节形成。有趣的是，观察到与磷酸盐和钙共固定的样品进一步增强。此外，在动物研究中，磷酸盐和钙共功能化的 PEEK 表现出显着增强的骨整合，与未功能化和磷酸盐功能化的 PEEK 相比，骨与种植体的直接接触比以及骨与种植体之间的粘合强度更大，这为 PEEK 的骨科和牙科应用铺平了道路。
Biomedical applications of poly(ether ether ketone) (PEEK) are hindered by its inherent bioinertness and lack of osseointegration capability. In the present study, to enhance osteogenic activity and, hence, the osseointegration capability of PEEK, we proposed a strategy of combined phosphate and calcium surface-functionalization, in which ozone-gas treatment and wet chemistry were used for introduction of hydroxyl groups and modification of phosphate and/or calcium, respectively. Surface functionalization significantly elevated the surface hydrophilicity without changing the surface roughness or topography. The cell study demonstrated that immobilization of phosphate or calcium increased the osteogenesis of rat mesenchymal stem cells compared with bare PEEK, including cell proliferation, alkaline phosphatase activity, and bone-like nodule formation. Interestingly, further enhancement was observed for samples co-immobilized with phosphate and calcium. Furthermore, in the animal study, phosphate and calcium co-functionalized PEEK demonstrated significantly enhanced osseointegration, as revealed by a greater direct bone-to-implant contact ratio and bond strength between the bone and implant than unfunctionalized and phosphate-functionalized PEEK, which paves the way for the orthopedic and dental application of PEEK.