Stress tolerance and antioxidant enzymatic activities in the metabolisms of the reactive oxygen species in two intertidal red algae Grateloupia turuturu and Palmaria palmata
Stress tolerance and antioxidant enzymatic activities in the metabolisms of the reactive oxygen species in two intertidal red algae Grateloupia turuturu and Palmaria palmata
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DOI：
10.1016/j.jembe.2009.11.005
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Pang, Shao Jun
中科院分区：
文献类型：
--
作者：
Liu, Feng;Pang, Shao Jun
本研究旨在比较两种相似的潮间带红海藻 Grateloupia turuturu Yamada 和 Palmaria palmata Kuntze 幼龄菌体的胁迫耐受性差异，并确定入侵藻 G. turuturu 是否比 A palmata 更能耐受不利环境条件的胁迫。为此，我们通过评估二氯氢荧光素（DCFH）氧化为二氯荧光素（DCF）、活性氧清除酶的活性以及当两个物种的菌体暴露于 添加MV、H2O2、3(3,4-二氯苯基)-1,1-二甲基脲(DCMU)、重金属、盐度变化、热和冷冻引起的氧化应激。结果表明，G. turuturu 的超氧化物歧化酶（SOD）和过氧化物酶（POD）活性远高于 P. palmata。 G. turuturu的Fv/Fm对MV、H2O2、DCMU、重金属、盐度和热应激的敏感性低于P. palmata，这表明G. turuturu可以更好地适应环境变化，因此比P. palmata具有更高的氧化应激阈值。 G. turuturu 对冷冻处理（-20 摄氏度）更敏感，这解释了为什么 G. turuturu 在较冷的水环境中很少出现。 (C) 2009 Elsevier B.V. 保留所有权利。
This investigation was designed to compare the differential stress tolerance in young thalli of two similar intertidal red seaweeds, Grateloupia turuturu Yamada and Palmaria palmata Kuntze, and to identify whether the invasive alga G. turuturu was more stress tolerant than A palmata to cope with adverse environmental conditions. To do so, we measured the production of reactive oxygen caused by methyl viologen (MV) by assessing the oxidation of dichlorohydrofluorescein (DCFH) to dichlorofluorescein (DCF), the activities of reactive oxygen scavenging enzymes and the changes of the optimal fluorescence quantum yield (Fv/Fm) when the thalli of the two species were exposed to oxidative stresses caused by the addition of MV, H2O2, 3 (3, 4-dichlorophenyl)-1,1-dimethyl urea (DCMU), heavy metal, changes of salinities, heat and freezing. Results demonstrated that the activities of superoxide dismutase (SOD) and peroxidase (POD) in G. turuturu were much higher than in P. palmata. Fv/Fm in G. turuturu was less sensitive than that in P. palmata to MV, H2O2, DCMU, heavy metal, salinity and heat stress, indicating that G. turuturu could be better acclimatized to changing environments and thus had a higher threshold for oxidative stress than P. palmata. G. turuturu was shown to be more sensitive to freezing treatment (-20 degrees C), which explained why the appearance of G. turuturu was rarely reported in colder water environments. (C) 2009 Elsevier B.V. All rights reserved.