Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice
Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice
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DOI：
10.3791/52434
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发表时间：
2015-02-01
影响因子：
1.2
通讯作者：
Wooten, Michael C.
中科院分区：
文献类型：
--
作者：
Seibenhener, Michael L.;Wooten, Michael C.
动物模型已被证明是非常宝贵的研究人员试图回答有关行为机制的问题。旷场迷宫是最常用的动物模型行为测量平台之一。这是一种快速且相对容易的测试，提供了从一般行走能力到关于受试动物情绪的数据的各种行为信息。由于它涉及啮齿动物模型，该程序允许研究实验室饲养和野生捕获的不同品系的小鼠或大鼠。该技术也容易适用于研究不同的药理学化合物的抗焦虑或致焦虑作用。在这里，一个协议使用的开放领域的迷宫来描述小鼠的行为进行了详细的和一个简单的分析一般的运动能力和焦虑相关的情绪行为之间的两个品系的C57 BL/6小鼠。简而言之，使用所描述的方案，我们显示野生型小鼠比年龄匹配的敲除小鼠表现出显著更少的焦虑相关行为，而两种品系表现出相似的行走能力。
Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of the most commonly used platforms to measure behaviors in animal models. It is a fast and relatively easy test that provides a variety of behavioral information ranging from general ambulatory ability to data regarding the emotionality of the subject animal. As it relates to rodent models, the procedure allows the study of different strains of mice or rats both laboratory bred and wild-captured. The technique also readily lends itself to the investigation of different pharmacological compounds for anxiolytic or anxiogenic effects. Here, a protocol for use of the open field maze to describe mouse behaviors is detailed and a simple analysis of general locomotor ability and anxiety-related emotional behaviors between two strains of C57BL/6 mice is performed. Briefly, using the described protocol we show Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.