Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences.
Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences.
复制标题
使用非同源 DNA 转化酿酒酵母：将转化 DNA 非法整合到酵母染色体中，以及将转化 DNA 与线粒体 DNA 序列体内连接。
DOI：
10.1128/mcb.13.5.2697-2705.1993
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发表时间：
1993
影响因子：
5.3
通讯作者：
Petes,TD
中科院分区：
文献类型：
--
作者：
Schiestl,RH;Dominska,M;Petes,TD
When the yeastSaccharomyces cerevisiaewas transformed with DNA that shares no homology to the genome, three classes of transformants were obtained. In the most common class, the DNA was inserted as the result of a reaction that appears to require base pairing between the target sequence and the terminal few base pairs of the transforming DNA fragment. In the second class, no such homology was detected, and the transforming DNA was integrated next to a CTT or GTT in the target; it is likely that these integration events were mediated by topoisomerase I. The final class involved the in vivo ligation of transforming DNA with nucleus-localized linear fragments of mitochondrial DNA.
