Evaluation of Porcine Psoas Major as a Scaffold Material for Engineered Heart Tissues
Evaluation of Porcine Psoas Major as a Scaffold Material for Engineered Heart Tissues
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DOI：
10.1089/ten.tec.2023.0064
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发表时间：
2023-08-10
影响因子：
3
通讯作者：
Campbell，Stuart G. G.
中科院分区：
文献类型：
--
作者：
Shen，Shi;Shao，Stephanie;Campbell，Stuart G. G.
脱细胞猪心肌通常用作工程心脏组织（EHT）的支架。然而，心肌的结构和机械异质性使机械一致组织的产生变得复杂。在这项研究中，我们评估了猪腰大肌（里脊肉）作为替代支架材料的效果。脱细胞里脊肉和心室支架的头对头比较显示，平均生物力学特征仅存在微小差异，但与心室样品相比，里脊肉支架的可变性较小，并且对起源区域的依赖性较小。通过接种里脊肉制成的 EHT 与含有人诱导多能干细胞来源的心肌细胞的心室支架制成的 EHT 的主动收缩行为也具有可比性，仅观察到微小差异。总的来说，数据表明，由脱细胞猪腰肌产生的 EHT 的行为与由猪左心室心肌产生的 EHT 的行为几乎相同，具有更均匀、生物力学一致且易于获得的优点。
Decellularized porcine myocardium is commonly used as scaffolding for engineered heart tissues (EHTs). However, structural and mechanical heterogeneity in the myocardium complicate production of mechanically consistent tissues. In this study, we evaluate the porcine psoas major muscle (tenderloin) as an alternative scaffold material. Head-to-head comparison of decellularized tenderloin and ventricular scaffolds showed only minor differences in mean biomechanical characteristics, but tenderloin scaffolds were less variable and less dependent on the region of origin than ventricular samples. The active contractile behavior of EHTs made by seeding tenderloin versus ventricular scaffolds with human-induced pluripotent stem cell-derived cardiomyocytes was also comparable, with only minor differences observed. Collectively, the data reveal that the behavior of EHTs produced from decellularized porcine psoas muscle is almost identical to those made from porcine left ventricular myocardium, with the advantages of being more homogeneous, biomechanically consistent, and readily obtainable.