The Limitations of an Exclusively Colloidal View of Protein Solution Hydrodynamics and Rheology
The Limitations of an Exclusively Colloidal View of Protein Solution Hydrodynamics and Rheology
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DOI：
10.1016/j.bpj.2013.10.012
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发表时间：
2013-11-19
影响因子：
3.4
通讯作者：
Pathak, Jai A.
中科院分区：
文献类型：
--
作者：
Sarangapani, Prasad S.;Hudson, Steven D.;Pathak, Jai A.
蛋白质是具有动态构象的复杂大分子。它们像胶体一样带电，但与胶体不同的是，电荷在它们的表面是不均匀分布的。在这里，我们推翻根深蒂固的学说，不加批判地对待牛血清白蛋白（BSA）作为一个胶体硬球通过阐明复杂的pH值和表面水合作用的依赖溶液粘度。我们测量了缓冲BSA溶液的无限剪切粘度，选择了一个参数空间来调整竞争的远程排斥和短程吸引（2mg /mL）
Proteins are complex macromolecules with dynamic conformations. They are charged like colloids, but unlike colloids, charge is heterogeneously distributed on their surfaces. Here we overturn entrenched doctrine that uncritically treats bovine serum albumin (BSA) as a colloidal hard sphere by elucidating the complex pH and surface hydration-dependence of solution viscosity. We measure the infinite shear viscosity of buffered BSA solutions in a parameter space chosen to tune competing long-range repulsions and short-range attractions (2 mg/mL