Sensitivity of CaM kinase II to the frequency of Ca2+ oscillations
Sensitivity of CaM kinase II to the frequency of Ca2+ oscillations
复制标题
DOI：
10.1126/science.279.5348.227
复制
发表时间：
1998-01-09
期刊：
影响因子：
56.9
通讯作者：
Schulman, H
中科院分区：
文献类型：
--
作者：
De Koninck, P;Schulman, H
许多细胞刺激的转导导致细胞内钙离子（Ca2+）浓度的振荡。虽然信息被认为是在这种振荡的频率编码，没有频率解码器已被确定。体外快速灌流固定化的钙离子和钙调素依赖性蛋白激酶II（CaM激酶II）表明，该酶可以解码的钙离子尖峰的频率到不同的激酶活性量。CaM激酶II的频率响应受几个因素的调制，包括单个尖峰的振幅和持续时间以及激酶的亚基组成和先前的激活状态。这些功能应提供特异性激活这种多功能酶的不同的细胞刺激，并可能成为其关键作用的活性依赖形式的突触可塑性。
The transduction of many cellular stimuli results in oscillations in the intracellular concentration of calcium ions (Ca2+). Although information is thought to be encoded in the frequency of such oscillations, no frequency decoder has been identified. Rapid superfusion of immobilized Ca2+ - and calmodulin-dependent protein kinase II (CaM kinase II) in vitro showed that the enzyme can decode the frequency of Ca2+ spikes into distinct amounts of kinase activity. The frequency response of CaM kinase II was modulated by several factors, including the amplitude and duration of individual spikes as well as the subunit composition and previous state of activation of the kinase. These features should provide specificity in the activation of this multifunctional enzyme by distinct cellular stimuli and may underlie its pivotal role in activity-dependent forms of synaptic plasticity.