Ontogeny of non-lymphoid and lymphoid cells in the rat gut with special reference to large mononuclear Ia-positive dendritic cells.
Ontogeny of non-lymphoid and lymphoid cells in the rat gut with special reference to large mononuclear Ia-positive dendritic cells.
复制标题
大鼠肠道中非淋巴细胞和淋巴细胞的个体发育，特别是大单核 Ia 阳性树突状细胞。
作者：
M. M. Wilders;T. Sminia;E. Janse
大鼠派尔集合淋巴结的个体发育进行了研究，特别是参考IA阳性树突状细胞。16日龄的胎儿含有大的IA阳性树突状细胞随机分布在整个肠壁和肠系膜。在从胎肠制备的细胞悬液中，这些细胞显示出典型的隐蔽细胞的运动模式，并且它们与这些和其他抗原呈递细胞共享超微结构特征。肠道被发现是第一个器官，其中大量的这些Ia阳性树突状细胞被发现。在妊娠16天，Ia阳性树突状细胞随机分布在整个原肠壁，但在后期阶段积累的结构，这是清楚地认识到从妊娠20天起的派尔氏斑。T和B淋巴细胞最早可在出生当天观察到，起初随机分布在派尔集合淋巴结中，但随后集中在单独的T细胞和B细胞区域。出生后两周，派尔集合淋巴结由密集的B细胞结节组成，主要包括表面IgM（sIgM）阳性细胞和结节间T细胞区域。IA阳性树突状细胞位于圆顶区的上皮细胞之间，就在松散的网状区域和结节间T细胞区域的上皮细胞下方。这些Ia阳性树突状细胞酸性磷酸酶阴性或仅弱阳性。一些Ia阳性树突状细胞也存在于小肠绒毛在所有年龄的研究。然而，这些细胞在出生后2周开始表达增加的酸性磷酸酶活性。因此，它们类似于巨噬细胞，而派尔斑树突状细胞显示出抗原呈递细胞的特征。
The ontogeny of rat Peyer's patches was studied with special reference to Ia-positive dendritic cells. The 16-day-old foetus contained large Ia-positive dendritic cells randomly distributed throughout the gut wall and mesentery. In cell suspensions prepared from the foetal gut, these cells showed the typical movement pattern of veiled cells, and they shared ultrastructural features with these and other antigen-presenting cells. The gut was found to be the first organ in which large numbers of these Ia-positive dendritic cells were found. At 16 days gestation, the Ia-positive dendritic cells were randomly distributed throughout the primitive gut wall, but at later stages accumulated in structures which were clearly recognizable as Peyer's patches from 20 days gestation onwards. T and B lymphocytes could be seen on the day of birth at the earliest, at first randomly distributed throughout the Peyer's patch, but afterwards concentrating in separate T- and B-cell regions. Two weeks after birth Peyer's patches consisted of densely populated B-cell nodules, mainly comprising surface IgM (sIgM) positive cells, and internodular T-cell regions. Ia-positive dendritic cells were situated between the epithelial cells of the dome area, just underneath this epithelium in a loose reticular area and in the internodular T-cell regions. These Ia-positive dendritic cells were acid phosphatase negative or only weakly positive. Some Ia-positive dendritic cells were also present in small intestinal villi at all ages studied. However, these cells started to express an increasing acid phosphatase activity in the 2 weeks following birth. Thus they resemble macrophages, while Peyer's patch dendritic cells show characteristics of antigen-presenting cells.