Nocardiopsis aegyptia sp nov., isolated from marine sediment
Nocardiopsis aegyptia sp nov., isolated from marine sediment
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DOI：
10.1099/ijs.0.02814-0
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发表时间：
2004-03-01
影响因子：
2.8
通讯作者：
Kroppenstedt, RM
中科院分区：
文献类型：
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作者：
Sabry, SA;Ghanem, NB;Kroppenstedt, RM
从埃及亚历山大港西部海岸Abu Qir湾的海洋沉积物中分离到一株放线菌SNG49(T)。该细菌为需氧革兰氏阳性。它产生米黄色到浅黄色的气生菌丝、棕色的底生菌丝和直到弯曲的菌丝，但没有特定的孢子链。16S rDNA序列分析和化学分类标记与Nocardiopsis属菌株SNG49(T)的分类一致，即中二氨基戊酸；无诊断糖；磷脂酰胆碱、磷脂酰甲基乙醇胺、磷脂酰肌醇、磷脂酰甘油和二磷脂酰甘油为极性脂类；MK-10系列(MK-10（H-0）至MK-10（H-8）的甲基萘醌；异/异支链脂肪酸和10-甲基支链脂肪酸，主要脂肪酸为异-17:0脂肪酸和结核硬脂酸。与菌株SNG49(T)在系统发育上有亲缘关系，分别具有98.8%和98.7%的16S rRNA基因序列相似性；然而，这两个物种与菌株SNG49(T)之间的DNA-DNA重关联值适中（分别为44%和60%），表明菌株SNG49(T)可以与它们明显分离。这些数据，连同不同的生理特征，得出结论，该分离物代表了诺卡opsis属中的一个新物种，因此提出了埃及诺卡opsis的名称。型应变为SNG49(T) (= DSM 44442（T) = NRRL B-24244(T)）。
An actinomycete, strain SNG49(T), was isolated from marine sediment of Abu Qir Bay, on the western seashore of Alexandria, Egypt. The bacterium was aerobic and Gram-positive. It produced beige to light-yellow aerial mycelium, brown substrate mycelium and straight to flexuous hyphae, but no specific spore chains. 16S rDNA sequence analysis and chemotaxonomic markers were consistent with classification of strain SNG49(T) in the genus Nocardiopsis, i.e. mesodiaminopimelic acid; no diagnostic sugars; phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol as polar lipids; menaquinones of the MK-10 series from MK-10(H-0) to MK-10(H-8); and iso/anteiso-branched and 10-methyl-branched fatty acids, the principal fatty acids being anteiso-17 : 0 and tuberculostearic acid. Nocardiopsis lucentensis and Nocardiopsis alba are the phylogenetic neighbours of strain SNG49(T), respectively showing 98.8 and 98.7% 16S rRNA gene sequence similarity; however, moderate DNA-DNA reassociation values between these two species and strain SNG49(T) (44 and 60%, respectively) showed that strain SNG49(T) could be clearly separated from them. These data, together with distinct physiological traits, led to the conclusion that this isolate represents a novel species within the genus Nocardiopsis, for which the name Nocardiopsis aegyptia is proposed. The type strain is SNG49(T) (= DSM 44442(T) = NRRL B-24244(T)).