Fine-needle aspiration cytology of peripheral T-cell lymphoma. A cytologic, immunologic, and cytometric study.
Fine-needle aspiration cytology of peripheral T-cell lymphoma. A cytologic, immunologic, and cytometric study.
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外周 T 细胞淋巴瘤的细针抽吸细胞学检查。
DOI：
10.1093/ajcp/91.2.120
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发表时间：
1989
影响因子：
3.5
通讯作者：
Butler,JJ
中科院分区：
文献类型：
--
作者：
Katz,RL;Gritsman,A;Cabanillas,F;Fanning,CV;Dekmezian,R;Ordonez,NG;Barlogie,B;Butler,JJ
外周T细胞淋巴瘤(PTCL)的诊断比较困难。本病可能被误诊为霍奇金氏病或非坏死性肉芽肿性淋巴结炎等反应性病变，也可能是淋巴瘤分类的问题。对13例经组织学证实的PTCL患者的细针抽吸物进行细胞学、免疫化学和流式细胞术检测。13例PTCL中，最初细胞学诊断为不典型淋巴细胞性浸润性淋巴瘤2例，混合细胞淋巴瘤6例，混合细胞淋巴瘤伴发组织细胞2例，大细胞淋巴瘤2例，小细胞淋巴瘤1例。对细胞旋体制剂进行表面标记研究。11例患者应用抗细胞毒抑制因子(Leu-2a)和辅助诱导物(Leu-3a，b)的抗体。10例淋巴瘤表现为辅助表型，1例表现为两个不同部位的表型异质性。PTCL最显著的细胞学特征是小、中、大淋巴样细胞的不同组合，核不规则，存在上皮样组织细胞和不典型的单核细胞。流式细胞术研究显示，尽管这种肿瘤具有临床侵袭性，但大多数病例中有二倍体干细胞系具有中等增殖活性(平均S期为6.7%)。
The diagnosis of peripheral T-cell lymphoma (PTCL) is difficult. This entity can be misdiagnosed as Hodgkin’s disease or a reactive process such as nonnecrotizing granulomatous lymphadenitis or it can present a problem in lymphoma classification. Fine-needle aspirates from 13 patients with histologically proven PTCL were evaluated by cytology, immunochemistry, and flow cytometry. Of the 13 patients with PTCL, initial cytologic diagnoses were atypical lymphocytic infiltrate (2), mixed-cell lymphoma (6), mixed-cell lymphoma with associated histiocytes (2), large cell lymphoma (2), and small cell lymphoma (1). Surface marker studies were performed on cytospin preparations. Antibodies against cytotoxic–suppressor (Leu-2a) and helper-inducer (Leu-3a,b) antigens were used in 11 cases. Ten lymphomas demonstrated helper phenotype and one showed phenotypic heterogeneity in two different sites. The most prominent cytologic features of PTCL were a variable combination of small, intermediate, and large lymphoid cells with irregular nuclei, presence of epithelioid histiocytes, and atypical mononuclear cells. Flow cytometry studies showed a diploid stem line with intermediate proliferative activity (mean S-phase of 6.7%) in most cases, despite the clinical aggressiveness of this neoplasm.