AFM Investigation on Ox-LDL-Induced Changes in Cell Spreading and Cell-Surface Adhesion Property of Endothelial Cells
AFM Investigation on Ox-LDL-Induced Changes in Cell Spreading and Cell-Surface Adhesion Property of Endothelial Cells
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AFM 对 Ox-LDL 诱导的内皮细胞细胞铺展和细胞表面粘附特性变化的研究
DOI：
10.1002/sca.21040
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发表时间：
2013-03-01
期刊：
影响因子：
--
通讯作者：
Chen, Yong
中科院分区：
文献类型：
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作者：
Shao, Wenxiang;Jin, Hua;Chen, Yong
内皮细胞的完整性和粘附性在动脉粥样硬化过程中起着至关重要的作用。氧化型低密度脂蛋白（Ox-LDL）影响内皮细胞的许多生理活动或力学性质。然而，Ox-LDL对内皮细胞的完整性和非特异性粘附特性的影响仍不清楚。本研究利用原子力显微镜（AFM）的形貌成像和力测量功能，发现Ox-LDL可通过破坏内皮细胞的细胞铺展性，暂时性地削弱内皮细胞的完整性，并通过破坏内皮细胞的非特异性粘附性，减少无关血细胞与内皮细胞的粘附。基于AFM的数据为了解Ox-LDL对内皮细胞的影响或在动脉粥样硬化形成过程中的作用提供了重要信息。扫描35：119-126，2013年。(c)2012 Wiley Periodicals，Inc.
The integrity and adhesion properties of endothelium play vital roles during atherosclerosis. It is well known that oxidized low-density lipoprotein (Ox-LDL) influences many physiological activities or mechanical properties of endothelial cells. However, the effects of Ox-LDL on the integrity and nonspecific adhesion properties of endothelial cells are still unclear. In this study, using the topographical imaging and force measurement functions of atomic force microscopy (AFM), we found that Ox-LDL can transiently weaken the integrity of endothelium by impairing cell spreading of endothelial cells and decrease the attachment of irrelevant blood cells to endothelium by impairing the nonspecific adhesion property of endothelial cells. The AFM-based data provide important information for understanding the effects of Ox-LDL on endothelial cells or during atherogenesis. SCANNING 35: 119-126, 2013. (c) 2012 Wiley Periodicals, Inc.