A Co-culture Assay to Determine Efficacy of TNF-α Suppression by Biomechanically Induced Human Bone Marrow Mesenchymal Stem Cells
A Co-culture Assay to Determine Efficacy of TNF-α Suppression by Biomechanically Induced Human Bone Marrow Mesenchymal Stem Cells
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DOI：
10.21769/bioprotoc.2513
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发表时间：
2017-08-20
期刊：
影响因子：
0.8
通讯作者：
Wenzel, Pamela L.
中科院分区：
文献类型：
--
作者：
Diaz, Miguel F.;Evans, Siobahn M.;Wenzel, Pamela L.
基于间充质干细胞（MSC）的细胞疗法的有益作用被认为主要由MSC抑制与慢性或急性损伤、感染、自身免疫和移植物抗宿主病相关的炎症的能力介导。为了明确血流引起的摩擦力或壁剪切应力（WSS）对人MSC免疫调节功能的影响，我们利用微流体技术对动脉腔壁处的WSS进行建模。随后通过在共培养测定中测量活化的鼠脾细胞的TNF-α产生来定量MSC的抗炎效力。TNF-α抑制测定用作MSC效力的功能评估的可再现平台，并证明作为MSC治疗功效的替代测定的预测值。
The beneficial effects of mesenchymal stem cell (MSC)-based cellular therapies are believed to be mediated primarily by the ability of MSCs to suppress inflammation associated with chronic or acute injury, infection, autoimmunity, and graft-versus-host disease. To specifically address the effects of frictional force caused by blood flow, or wall shear stress (WSS), on human MSC immunomodulatory function, we have utilized microfluidics to model WSS at the luminal wall of arteries. Anti-inflammatory potency of MSCs was subsequently quantified via measurement of TNF-alpha production by activated murine splenocytes in co-culture assays. The TNF-alpha suppression assay serves as a reproducible platform for functional assessment of MSC potency and demonstrates predictive value as a surrogate assay for MSC therapeutic efficacy.