THE INTERACTION OF PROTEINS WITH HYDROXYAPATITE .3. MECHANISM
THE INTERACTION OF PROTEINS WITH HYDROXYAPATITE .3. MECHANISM
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DOI：
10.1016/0003-2697(84)90241-0
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发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
TIMASHEFF, SN
中科院分区：
文献类型：
--
作者：
GORBUNOFF, MJ;TIMASHEFF, SN
建立了蛋白质与羟基磷灰石（HA）结合和洗脱的机理。通过蛋白质正电荷与HA之间的非特异性吸引和蛋白质羧基与矿物上Ca位点的特异性络合，结合发生。洗脱可以作为电荷的非特异性离子屏蔽的结果，也可以通过蛋白质基团从与其络合的柱上的位点的特异性置换而发生。
The mechanism of protein binding to and elution from hydroxyapatite (HA) was established. Binding occurs both by nonspecific attraction between protein positive charges and HA and by specific complexing of protein carboxyls with Ca loci on the mineral. Elution can take place either as the result of the nonspecific ion screening of charges or by the specific displacement of protein groups from sites on the column with which they had complexed.