T cell infiltration after chronic constriction injury of mouse sciatic nerve is associated with interleukin-17 expression
T cell infiltration after chronic constriction injury of mouse sciatic nerve is associated with interleukin-17 expression
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DOI：
10.1016/j.expneurol.2006.03.014
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发表时间：
2006-08-01
影响因子：
5.3
通讯作者：
Stoll, Guido
中科院分区：
文献类型：
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作者：
Kleinschnitz, Christoph;Hofstetter, Harald H.;Stoll, Guido
白细胞介素17A(IL-17A)是最近发现的一种新的T细胞细胞因子，可在多种免疫介导性疾病中协调炎症反应。本研究采用定量逆转录聚合酶链式反应(qRT-PCR)技术，分析了小鼠坐骨神经慢性压迫性损伤(CCI)后IL-17A及其主要调控因子IL-23和IL-15的表达模式。在CCI后第7天，IL-17A在变性神经中呈单相表达，而IL-17A调节细胞因子IL-23和IL-15的转录较早达到峰值。相应地，免疫细胞化学显示损伤神经内膜内可检测到IL-17A阳性T细胞。为了支持T细胞炎症的关键作用，缺乏功能性T淋巴细胞的RAG-1基因敲除小鼠在CCI后的远端神经节段不表达IL-17A mRNA。有趣的是，T细胞缺陷与CCI后热痛觉过敏减轻以及巨噬细胞标志分子F4/80和趋化因子巨噬细胞趋化蛋白-1(MCP-1)的mRNA水平降低有关。我们的研究支持T细胞和T细胞衍生的细胞因子参与周围神经损伤后的炎症反应的观点。(C)2006 Elsevier Inc.保留所有权利。
Interleukin (IL)-17A, a recently described novel T cell cytokine, orchestrates inflammation in a variety of immune-mediated diseases. In the present investigation, we analyzed the temporal gene expression pattern of IL-17A and its main regulators IL-23 and IL-15 after chronic constriction injury (CCI) of the sciatic nerve, a lesion paradigm inducing neuropathic pain, by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in mice. IL-17A displayed a monophasic expression in degenerating nerves at day 7 after CCI while transcripts for the IL-17A regulatory cytokines IL-23 and IL-15 peaked earlier. Accordingly, IL-17A positive T cells were detectable within the endoneurium of the injured nerves by immunocytochemistry. In support of a crucial role of T cell inflammation, RAG-1 knockout mice lacking functional T lymphocytes did not express IL-17A mRNA in distal nerve segments following CCI. Interestingly, T cell deficiency was associated with less thermal hyperalgesia and reduced mRNA levels for the macrophage marker molecule F4/80 and the chemokine macrophage chemoattractant protein-1 (MCP-1) after CCI. Our study supports the notion that T cells and T-cell-derived cytokines contribute to the inflammatory response after peripheral nerve injury. (c) 2006 Elsevier Inc. All rights reserved.