Gene expression profile during proliferation and differentiation of rainbow trout adipocyte precursor cells.
Gene expression profile during proliferation and differentiation of rainbow trout adipocyte precursor cells.
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DOI：
10.1186/s12864-017-3728-0
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发表时间：
2017-05-04
期刊：
影响因子：
4.4
通讯作者：
Navarro I
中科院分区：
文献类型：
--
作者：
Bou M;Montfort J;Le Cam A;Rallière C;Lebret V;Gabillard JC;Weil C;Gutiérrez J;Rescan PY;Capilla E;Navarro I
养殖鱼类脂肪组织的过度积累是水产养殖中的一个突出问题。要了解肥胖的发展，关键是要确定哪些基因的表达与成脂分化。因此，在不同的时间点（第3，8，15和21天）沿着原代培养虹鳟鱼前脂肪细胞的发展的转录谱已被调查使用安捷伦鳟鱼寡核苷酸微阵列。我们的分析确定了4026个差异表达的基因（倍数变化>3），这些基因被分为两个主要簇，对应于前脂肪细胞培养期间观察到的主要阶段：增殖和分化。增殖簇包含1028个从培养第3天至第8天上调的基因，而分化簇的特征在于2140个从培养第15天至第21天诱导的基因。增殖的特征在于参与基本细胞和代谢过程（转录、核糖体生物发生、翻译和蛋白质折叠）、细胞重塑和自噬的基因的富集。此外，类花生酸信号通路的影响在这一阶段得到了强调。另一方面，终末分化阶段富含参与能量产生、脂质和碳水化合物代谢的基因。此外，在该阶段，参与脂滴形成的基因的富集以及甲状腺受体/视黄酸X受体（TR/RXR）和过氧化物酶体增殖物激活受体（PPARs）信号传导途径的激活被证明。整个脂肪形成过程是由转录因子和表观遗传调节因子的协调激活驱动的。总之，我们的研究表明，在虹鳟鱼脂肪细胞增殖和分化过程中，功能相关基因的协调表达。此外，所产生的信息将允许未来的调查参与特定阶段的鱼类脂肪形成的特定基因。本文的在线版本（doi：10.1186/s12864-017-3728-0）包含补充材料，可供授权用户使用。
Excessive accumulation of adipose tissue in cultured fish is an outstanding problem in aquaculture. To understand the development of adiposity, it is crucial to identify the genes which expression is associated with adipogenic differentiation. Therefore, the transcriptomic profile at different time points (days 3, 8, 15 and 21) along primary culture development of rainbow trout preadipocytes has been investigated using an Agilent trout oligo microarray. Our analysis identified 4026 genes differentially expressed (fold-change >3) that were divided into two major clusters corresponding to the main phases observed during the preadipocyte culture: proliferation and differentiation. Proliferation cluster comprised 1028 genes up-regulated from days 3 to 8 of culture meanwhile the differentiation cluster was characterized by 2140 induced genes from days 15 to 21. Proliferation was characterized by enrichment in genes involved in basic cellular and metabolic processes (transcription, ribosome biogenesis, translation and protein folding), cellular remodelling and autophagy. In addition, the implication of the eicosanoid signalling pathway was highlighted during this phase. On the other hand, the terminal differentiation phase was enriched with genes involved in energy production, lipid and carbohydrate metabolism. Moreover, during this phase an enrichment in genes involved in the formation of the lipid droplets was evidenced as well as the activation of the thyroid-receptor/retinoic X receptor (TR/RXR) and the peroxisome proliferator activated receptors (PPARs) signalling pathways. The whole adipogenic process was driven by a coordinated activation of transcription factors and epigenetic modulators. Overall, our study demonstrates the coordinated expression of functionally related genes during proliferation and differentiation of rainbow trout adipocyte cells. Furthermore, the information generated will allow future investigations of specific genes involved in particular stages of fish adipogenesis. The online version of this article (doi:10.1186/s12864-017-3728-0) contains supplementary material, which is available to authorized users.