The complete mitochondrial genome of Choristoneura longicellana (Lepidoptera: Tortricidae) and phylogenetic analysis of Lepidoptera
The complete mitochondrial genome of Choristoneura longicellana (Lepidoptera: Tortricidae) and phylogenetic analysis of Lepidoptera
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DOI：
10.1016/j.gene.2016.07.003
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发表时间：
2016-10-10
期刊：
影响因子：
3.5
通讯作者：
Zhu, Chao-Dong
中科院分区：
文献类型：
--
作者：
Wu, Yu-Peng;Zhao, Jin-Liang;Zhu, Chao-Dong
为了更好地了解鳞翅目昆虫的多样性和进化史，测定了长节卷蛾Choristoneura longicellana（=长节星蛾Hoshinoa longicellana）的线粒体全基因组。它是一个典型的环状双链体分子，长度为15，759 bp，包含13个蛋白质编码基因，22个转运RNA基因，2个核糖体RNA基因和一个富含A + T的区域。所有推测的tRNA二级结构都显示出共同的三叶草模式，除了trnS 1（AGN），它缺少DHU臂。Longicellana是已测序的鳞翅目昆虫中最长的。C. Longicellana与GenBank中所有鳞翅目昆虫的基因序列相同。结果表明，该基因有5个重叠区，范围在1 ~ 8bp之间，有14个间隔区，范围在1 ~ 48 bp之间。此外，在C. A + T富集区还存在4个相似的串联宏卫星区，长度分别为101、98、92和92 bp。longicellana通过对13个总科89种鳞翅目昆虫的抽样调查，运用贝叶斯推断和最大似然分析方法，重建了鳞翅目昆虫之间的亲缘关系。两个系统发育树的拓扑结构基本一致，但由于有限的样本量，除了丽藻总科位于不同的地理位置外，丽藻总科、粪形总科、盖藻总科、复盖藻总科的位置均未确定。（Geometroidea +（Noctuoidea + Eschercoidea））构成大鳞翅目的“核心”。Pyraloidea组与“核心”Macrolepidoptera。凤蝶总科不是大鳞翅目。弄蝶科（代表弄蝶总科）是蝶总科的一个巢，与粉蝶科和凤蝶科关系密切。本文恢复了（蛱蝶科+（斑蝶科+灰蝶科））的关系。(C)2016爱思唯尔B. V.保留所有权利。
To better understand the diversity and phylogeny of Lepidoptera, the complete mitochondria] genome of Choristoneura longicellana (= Hoshinoa longicellana) was determined. It is a typical circular duplex molecule with 15,759 bp in length, containing the standard metazoan set of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and an A + T-rich region. All of the inferred tRNA secondary structures show the common cloverleaf pattern, with the exception of trnS1(AGN), which lacks the DHU arm. The rrnL of C. Longicellana is the longest in sequenced lepidopterans. C. Longicellana has the same gene order as all lepidopteran species currently available in GenBank. There are 5 overlapping regions ranging from 1 bp to 8 bp and 14 intergenic spacers ranging from 1 bp to 48 bp. In addition, there are four similar tandem macro-satellite regions with the lengths of 101 bp, 98 bp, 92 bp, and 92 bp respectively in the A + T-rich regions of C. longicellana. We sampled 89 species representing 13 superfamilies, and reconstructed their relationship among Lepidoptera by Bayesian Inference and Maximum Likelihood analysis. The topology of the two phylogenetic analysis trees is identical roughly, except for Cossoidea in different locations, the positions of Cossoidea, Copromorphoidea, Gelechioidea, Zygaenoidea were not determined based the limited sampling. (Geometroidea + (Noctuoidea + Bombycoidea)) form the Macrolepidoptera "core". Pyraloidea group with the "core" Macrolepidoptera. Papilionoidea are not Macrolepidoptera. The Hesperiidae (represent Hesperioidea) is nested in the Papilionoidea, and closely related to Pieridae and Papilionidae. The well-known relationship of (Nymphalidae + (Riodinidae + Lycaenidae)) is recovered in this paper. (C) 2016 Elsevier B.V. All rights reserved.