Atmospheric cold plasma treatment of soybean protein isolate: insights into the structural, physicochemical, and allergenic characteristics
Atmospheric cold plasma treatment of soybean protein isolate: insights into the structural, physicochemical, and allergenic characteristics
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大豆分离蛋白的常压冷等离子体处理：深入了解结构、理化和过敏特性
DOI：
10.1111/1750-3841.15556
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发表时间：
2021
影响因子：
3.9
通讯作者：
Fu Linglin
中科院分区：
文献类型：
--
作者：
Zhang Qiaozhi;Cheng Zhouzhou;Zhang Jianhao;Nasiru Mustapha Muhammad;Wang Yanbo;Fu Linglin
目前，人们对揭示血浆和食品基质之间的相互作用的兴趣激增。在本研究中，我们研究了常压冷等离子体(ACP)处理对大豆分离蛋白(SPI)结构、理化和致敏特性的影响。用不同频率(80~100 Hz)和不同持续时间(1~10min)的ACP处理SPI分散体，研究暴露条件对SPI分散体的影响。结果表明，ACP可诱导大豆蛋白氧化，导致大豆分离蛋白二级和三级结构发生改变。结果表明，大豆分离蛋白的乳化性能(与对照相比为56%~168%)和起泡性能(60%~194%)均受到不同程度的影响。此外，在一定的条件下(120赫兹，5min)，SPI的IgE结合水平比对照组降低了75%。适度处理产生的产品具有更好的功能性和降低的过敏性，而过度暴露则由于蛋白质聚集而导致可销售性的丧失。实际应用在本研究中，我们首次证明了血浆物种与大豆蛋白发生反应，导致空间结构的变化，这与蛋白质的功能和过敏性密切相关。ACP与水体系中的大分子相互作用，因此可以作为一种替代的、有前途的非热改性大豆蛋白的方法，但不同工艺参数的确切作用还需要很好地阐述。
Currently, there has been a surge of interest in revealing the interactions between plasma and food matrices. In this study, we investigated the impacts of atmospheric cold plasma (ACP) treatment on the structural, physicochemical and allergenic characteristics of soybean protein isolate (SPI). SPI dispersions were subjected to ACP treatments at different frequencies (80 to 100 Hz) and durations (1 to 10 min) to investigate the effects of exposing conditions. Results showed that ACP induced reactive oxygen species-mediated oxidation of soy proteins, resulting in modifications in the secondary and ternary structures of SPI. As a consequence, functional properties of SPI, such as emulsifying (56 to 168%, compared with control) and foaming properties (60 to 194%) were influenced by varying degrees. In addition, under certain circumstance (120 Hz, 5 min), the IgE-binding level of SPI was decreased by up to 75%, when compared to the control. Moderate treatment yielded products with improved functionality and reduced allergenicity, while extensive exposure induced a loss of vendibility due to protein aggregation. Practical Application In this study, we demonstrated for the first time, that plasma species reacted with soybean proteins, resulting in spatial structural changes which are closely related with protein functionality and allergenicity. ACP interacts with macromolecules in aqueous systems and thus can be an alternative and promising nonthermal approach in modifying soybean proteins, whereas the exact role of different processing parameters needs to be well-elaborated.