Ternary Emission of a Blue-, Green-, and Red-Based Molecular Imprinting Fluorescence Sensor for the Multiplexed and Visual Detection of Bovine Hemoglobin
Ternary Emission of a Blue-, Green-, and Red-Based Molecular Imprinting Fluorescence Sensor for the Multiplexed and Visual Detection of Bovine Hemoglobin
复制标题
用于牛血红蛋白多重视觉检测的蓝色、绿色和红色分子印迹荧光传感器的三元发射
DOI：
10.1021/acs.analchem.9b00082
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发表时间：
2019
影响因子：
7.4
通讯作者：
Chen Lingxin
中科院分区：
文献类型：
--
作者：
Yang Qian;Li Jinhua;Wang Xiaoyan;Xiong Hua;Chen Lingxin
将牛血红蛋白（BHb）印迹聚合物（b-MIPs、g-MIPs和r-MIPs）按一定比例混合后，制备了一种新型的三发射荧光传感器，实现了BHb的多路视觉检测。这三种MIPs分别嵌入蓝色发射的7-羟基香豆素量子点、绿色发射的CdTe量子点和红色发射的CdTe/ZnS量子点。与BHb相互作用8 min后，CdTe和CdTe/ZnS量子点的荧光同时关闭，而7-羟基香豆素则开启荧光强度。因此，三元发射的比例荧光强度在0.025 ~ 3 μ BHb范围内呈线性变化，荧光颜色从黄绿色→黄色→橙红色→李子色→紫色→最后的蓝色。与双发射或单发射传感器相比，三发射荧光MIPs传感器的颜色变化范围更广，覆盖绿-红-蓝窗口，可用于肉眼准确测定BHb，检测限低至7.8 nM，印迹因子高至15.2。此外，在牛尿液样品中加标BHb的检测回收率为99.25 ~ 111.7%，光学稳定性和印迹后构建的便便性表明，所开发的三色发射荧光MIPs传感器为复杂样品中蛋白质的快速、敏感和视觉检测提供了理想的选择。
A novel ternary-emission fluorescence sensor was proposed by post-imprinting mixing blue-/green-/red-emission bovine hemoglobin (BHb) imprinted polymers (b-MIPs, g-MIPs, and r-MIPs) at a proper ratio and realized the multiplexed and visual detection of BHb. The three MIPs were individually embedded with blue-emission 7-hydroxycoumarin, green-emission CdTe quantum dots (QDs), and red-emission CdTe/ZnS QDs. Upon interaction with BHb within 8 min, the fluorescence of CdTe and CdTe/ZnS QDs were simultaneously turned off, whereas the 7-hydroxycoumarin turned on the fluorescence intensity. Thereupon, the ratiometric fluorescence intensity of the ternary emission linearly varied within 0.025–3 μM BHb, accompanying the profuse fluorescence color evolution from yellowish green to yellow to salmon to plum to purple to finally blue. In comparison with the dual- or single-emission sensor, the ternary-emission fluorescence MIPs sensor provided a wider color variation covering the green–red–blue window for accurate naked-eye determination of BHb, as well as a lower detection limit down to 7.8 nM and a higher imprinting factor of 15.2. Moreover, the satisfactory recoveries of 99.25–111.7% in determining the spiked BHb in bovine urine samples, as well as the optical stability and post-imprinting construction convenience, indicated that the developed tricolor-emission fluorescence MIPs sensor provided an ideal alternative for rapid, sensitive, and visual determination of proteins in complicated samples.