AMINO-ACID-SEQUENCE OF HUMAN PROTEIN-Z, A VITAMIN-K-DEPENDENT PLASMA GLYCOPROTEIN
AMINO-ACID-SEQUENCE OF HUMAN PROTEIN-Z, A VITAMIN-K-DEPENDENT PLASMA GLYCOPROTEIN
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DOI：
10.1016/0006-291x(90)91566-b
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发表时间：
1990-11-15
影响因子：
3.1
通讯作者：
DAVIE, EW
中科院分区：
文献类型：
--
作者：
ICHINOSE, A;TAKEYA, H;DAVIE, EW
蛋白Z是从人和牛血浆中分离和鉴定的一种依赖维生素K的糖蛋白。通过从肝脏文库中分离噬菌体克隆和另外两个肝脏文库的体外扩增，获得了人类蛋白Z的编码基因。蛋白Z是由40个氨基酸组成的前前体序列合成的。该成熟蛋白由360个残基组成，包括一个由13个羧基谷氨酸残基组成的GLA结构域，两个表皮生长因子结构域，以及一个与丝氨酸蛋白酶催化结构域高度同源的羧基末端区域。然而，人蛋白Z在活性中心的催化三联体中含有天冬氨酸而不是丝氨酸和赖氨酸而不是组氨酸。
Protein Z is a vitamin K-dependent glycoprotein isolated and characterized from human and bovine plasma. A cDNA coding for human protein Z has been obtained by the isolation of phage clones from a liver cDNA library and in vitro amplification of two other liver libraries. Protein Z is synthesized with a prepro-leader sequence of 40 amino acids. The mature protein is composed of 360 residues including a Gla domain of 13 carboxyglutamic acid residues, two epidermal growth factor domains, and a carboxyl terminal region which is highly homologous to the catalytic domain of serine proteases. Human protein Z, however, contains an Asp instead of Ser and a Lys instead of His in the catalytic triad of the active site.