Genetic structure of populations of the green turtle (Chelonia mydas) in Japan using mtDNA control region sequences
Genetic structure of populations of the green turtle (Chelonia mydas) in Japan using mtDNA control region sequences
复制标题
使用 mtDNA 控制区序列研究日本绿海龟 (Chelonia mydas) 种群的遗传结构
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
H. Koike
中科院分区：
文献类型：
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作者：
T. Hamabata;S. Nishida;N. Kamezaki;H. Koike
绿色海龟？7zydos分布于世界热带和热带海洋。本种的最适种群日本种群由于产卵海滩的损失而减少，沿海种群为3。为了研究日本群体的遗传结构3Rd遗传多样性，对来自两个试验点的20个个体和294个日本觅食地的个体进行了肌肉和皮肤样品的遗传多样性分析。用特异性引物对日本猪线粒体PNA（mtDNA）的cofttrsi区进行扩增，从日本猪群体中检测到32种单倍型。因此，日本列岛的模式多样性（k）（八重山为0.89，奄美大尻为0.83）高于本岛（Nom 3 ike，Meirsto，3Rd Owase分别为0.69，e.63和e.67）。使用32个单倍型的xnedi 3 n-joiniRg树显示了三个分支，并且主导单倍型位于每个分支的中部。对5个地区单倍型频率分布的地理异质性进行了精确检验和FsT检验，结果表明，八重岩3号与主要的13个地区之间存在显著差异，奄美大岛与野梅池/尾濑31个地区之间存在显著差异。
The green turtle Chelonia ?7zydos is distributed threughout tropical and sgbtrepic31 oce3iks worldwide. The Japanese popt{lation, the itorthemmest popu}atioR ef this species, is decreasing d"e to loss ef spawniftg beaches, aRd peopling to the coastai are3. To examiRe the genetiÅí structure 3Rd genetic diversity of the Japanese population, a total of 294 individuals from seveR foraging }ocaiities aRd 20 iRdividgials frem two ftesting sites were awalyzed usiRg muscle and skiR samples. The cofttrsi region of rnitochondriai PNA (mtDNA) was amplified with specific primers, aRd 32 haplotypes were detected from the Jap3Rese pgpulatiork. }Ilapletype diversities (k) of the soEgthern isiands (O.89 at Yaeyama aftd O.83 at Amami-Ohshirif}a) were higker thaR those ofthe reain islands (O.69, e.63, and e.67 at Nom3ike, Meirsto, 3Rd Owase, respectively). A xnedi3n-joiniRg tree using the 32 haplotypes showed three elades, and domimant h3piotypes were situated iR the midd}e of each clade. The exact test and FsT for geegraphic heteregeiteity in haplotype frequency distribvitioRs fgr the five feraging localities shewed that sigRificaRt differences were detected between Yaeyam3 and the maiR isl3nd localities, aRd between the AmamiOhshima aRd Nomaike/Owase lec31ities.