In vivo evidence of enhanced di-methylation of histone H3 K4 on upregulated genes in adipose tissue of diabetic db/db mice
In vivo evidence of enhanced di-methylation of histone H3 K4 on upregulated genes in adipose tissue of diabetic db/db mice
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DOI：
10.1016/j.bbrc.2010.11.097
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发表时间：
2011-01-07
影响因子：
3.1
通讯作者：
Mochizuki, Kazuki
中科院分区：
文献类型：
--
作者：
Fujimoto, Saki;Goda, Toshinao;Mochizuki, Kazuki
组蛋白H3赖氨酸（K）4的二甲基化是表观遗传记忆的一个组成部分，与基因反式激活有关。在这项研究中，我们检测了糖尿病的发展是否会诱导上调基因上组蛋白H3 1 β的去甲基化。我们使用微阵列分析，与非糖尿病db/m小鼠相比，在胰岛素抵抗/糖尿病db/db小鼠的肠系膜脂肪组织中寻找上调基因。我们还对db/m和db/db小鼠肠系膜脂肪组织中上调基因中组蛋白H3 1 β 4的二甲基化进行了染色质免疫沉淀分析。与db/m小鼠相比，db/db小鼠肠系膜脂肪组织中Atp 6v 0 d2、Mmp 12、Trem 2和Clec 4d基因上游和/或转录区组蛋白H3 K4的二甲基化水平升高。这些结果表明，组蛋白H3 1 β 4的去甲基化参与了db/db小鼠肠系膜脂肪组织中Atp 6 v0 d2、Mmp 12、Trem 2和Clec 4d的诱导。(C)2010年爱思唯尔公司All rights reserved.
Di-methylation of histone H3 lysine (K) 4, a component of the epigenetic memory, is associated with gene transactivation. In this study, we examined whether the development of diabetes induces di-methylation of histone H3 1(4 on the upregulated genes. We searched for upregulated genes in mesenteric adipose tissue of insulin-resistant/diabetic db/db mice compared with non-diabetic db/m mice using microarray analysis. We also performed chromatin immunoprecipitation assays for di-methylation of histone H3 1(4 in the upregulated genes in mesenteric adipose tissue of db/m and db/db mice. Di-methylation of histone H3 K4 was enhanced at the upstream and/or transcribed regions of upregulated genes including Atp6v0d2, Mmp12, Trem2 and Clec4d genes in mesenteric adipose tissue of db/db mice, as compared with db/m mice. These results suggest that di-methylation of histone H3 1(4 is involved in the induction of Atp6v0d2, Mmp12, Trem2 and Clec4d in mesenteric adipose tissue in db/db mice. (C) 2010 Elsevier Inc. All rights reserved.