Graded effect of tailless on posterior gut development: molecular basis of an allelic series of a nuclear receptor gene.
Graded effect of tailless on posterior gut development: molecular basis of an allelic series of a nuclear receptor gene.
复制标题
无尾对后肠道发育的分级效应：核受体基因等位基因系列的分子基础。
DOI：
10.1016/0925-4773(95)00467-x
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发表时间：
1996
影响因子：
2.6
通讯作者：
Lengyel,JA
中科院分区：
文献类型：
--
作者：
Diaz,RJ;Harbecke,R;Singer,JB;Pignoni,F;Janning,W;Lengyel,JA
通过标记早期原肠胚阶段胚胎的细胞，我们发现在强tll等位基因突变的胚胎中，命运图向后移动，后肠原基被删除。因此，我们使用后肠发育的方面来表征新的和先前描述的tll等位基因的表型。在胚胎突变的各种等位基因，胚盘表达的Trg（T-相关基因，需要建立后肠）和成熟后肠大小的相对水平进行了测定，这些测定的结果相互关联。在映射到编码无尾核受体蛋白的序列的等位基因中，所有（四个）影响DNA结合结构域的锌指;令人惊讶的是，高度保守残基的取代允许如通过我们的生物测定检测到的一系列活性。
By marking cells of early gastrula stage embryos, we showed that in embryos mutant for a strong tll allele the fate map is shifted posteriorly and the hindgut anlage is deleted. We therefore used aspects of hindgut development to characterize the phenotype of new and previously described tll alleles. In embryos mutant for the various alleles, relative levels of blastoderm expression of Trg (T-related gene, required to establish the hindgut) and of mature hindgut size were determined; the results of these assays correlated with each other. Of the alleles that map to the sequence encoding the Tailless nuclear receptor protein, all (four) affect the zinc fingers of the DNA binding domain; surprisingly, substitutions of highly conserved residues allow a range of activities as detected by our bioassays.