SINGLE-CRYSTALS OF V-AMYLOSE COMPLEXED WITH ISOPROPANOL AND ACETONE
SINGLE-CRYSTALS OF V-AMYLOSE COMPLEXED WITH ISOPROPANOL AND ACETONE
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DOI：
10.1016/0141-8130(90)90078-o
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发表时间：
1990-02-01
影响因子：
8.2
通讯作者：
CHANZY, H
中科院分区：
文献类型：
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作者：
BULEON, A;DELAGE, MM;CHANZY, H
将这些沉淀剂加入到直链淀粉的亚稳水溶液中，制备了与异丙醇或丙酮络合的直链淀粉单晶。两种沉淀剂均可获得微米大小的血小板晶体。他们给出了难以区分的电子衍射图，可以在正交晶胞中索引，a = 28.26 . ang。， b = 29.30 . ang。， c = 8.01 . ang。在P212121或P21212空间组中。在单位细胞内，直链淀粉链以反平行的65条直链螺旋排列，占细胞含量的70%，其余30%由异丙醇/丙酮和水组成，估计每个单位细胞有10个异丙醇/丙酮分子和52个水分子。如果晶体在不同温度下悬浮在纯异丙醇中或在室温下悬浮在纯甲醇中，它们会经历一个脱溶剂过程，最终将其转化为VH直链淀粉。异丙醇的脱溶剂作用使晶体保持完整，而甲醇的脱溶剂作用使晶体在收缩过程中开裂。对这种差异提出了一种解释。
Single crystals of amylose complexed with isopropanol or acetone were prepared by adding these precipitants to a metastable aqueous solution of amylose. With both precipitants, similar micrometre sized platelet crystals were obtained. They gave indistinguishable electron diffraction diagrams which could be indexed in orthorhombic unit cell, with a = 28.26 .ANG., b = 29.30 .ANG., c = 8.01 .ANG. and in a space group P212121 or P21212. Within the unit cell, the amylose chains are organized in antiparallel pairs of parallel 65 amylose helices occupying 70% of the cell content, the remaining 30% consisting of isopropanol/acetone and water, with an estimate of 10 isopropanol/acetone molecules for 52 water molecules per unit cell. If the crystals are suspended in pure isopropanol at various temperatures or in pure methanol at room temperature, they undergo a de-solvation process that ultimately converts them into VH amylose. De-solvation with isopropanol left the crystals intact whereas the methanol, they became cracked during shrinkage. An explanation is proposed for such difference.