Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3+ T regulatory cells.
Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3+ T regulatory cells.
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DOI：
10.4049/jimmunol.0904028
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发表时间：
2010-04-01
期刊：
影响因子：
--
通讯作者：
Shevach EM
中科院分区：
文献类型：
--
作者：
Thornton AM;Korty PE;Tran DQ;Wohlfert EA;Murray PE;Belkaid Y;Shevach EM
Helios是Ikaros转录因子家族的成员，其优先在mRNA水平上由调节性T细胞（Treg细胞）表达。我们使用新产生的mAb评估了Helios蛋白的表达，并证明其在胸腺发育的双阴性2阶段在所有胸腺细胞中表达。尽管Helios在100%的CD 4 + CD 8 − Foxp 3+胸腺细胞中表达，但其在外周淋巴组织中的表达仅限于小鼠和人类Foxp 3 + T细胞亚群（约70%）。在TGF-β表达的Helios存在下，小鼠或人初始T细胞均未通过TCR刺激在体外诱导表达Foxp 3。通过Ag喂养在体内诱导的Ag特异性Foxp 3 + T细胞也未能表达Helios。总的来说，这些结果表明Helios可能是胸腺来源的Treg细胞的特异性标志物，并提高了显著百分比的Foxp 3 + Treg细胞在胸腺外产生的可能性。
Helios, a member of the Ikaros transcription factor family, is preferentially expressed at the mRNA level by regulatory T cells (Treg cells). We evaluated Helios protein expression using a newly generated mAb and demonstrated that it is expressed in all thymocytes at the double negative 2 stage of thymic development. Although Helios was expressed by 100% of CD4+CD8−Foxp3+ thymocytes, its expression in peripheral lymphoid tissues was restricted to a subpopulation (~70%) of Foxp3+ T cells in mice and humans. Neither mouse nor human naive T cells induced to express Foxp3 in vitro by TCR stimulation in the presence of TGF-β expressed Helios. Ag-specific Foxp3+ T cells induced in vivo by Ag feeding also failed to express Helios. Collectively, these results demonstrate that Helios is potentially a specific marker of thymic-derived Treg cells and raises the possibility that a significant percentage of Foxp3+ Treg cells are generated extrathymically.
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影响因子：
15.3
作者：
Apostolou, I;von Boehmer, H
通讯作者：
von Boehmer, H
DOI：
10.1084/jem.20081359
发表时间：
2008-12-22
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
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影响因子：
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作者：
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影响因子：
32.4
作者：
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通讯作者：
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影响因子：
32.4
作者：
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通讯作者：
Hsieh, Chyi-Song