A novel electrochemical immunosensor based on catalase functionalized AuNPs-loaded self-assembled polymer nanospheres for ultrasensitive detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether
A novel electrochemical immunosensor based on catalase functionalized AuNPs-loaded self-assembled polymer nanospheres for ultrasensitive detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether
复制标题
一种基于过氧化氢酶功能化 AuNPs 的自组装聚合物纳米球的新型电化学免疫传感器，用于超灵敏检测四溴双酚 A 双（2-羟乙基）醚
DOI：
10.1016/j.aca.2018.10.018
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发表时间：
2019
影响因子：
6.2
通讯作者：
Zhang Zhen
中科院分区：
文献类型：
--
作者：
Dong Shuaibing;Wang Shaochen;Gyimah Eric;Zhu Nuanfei;Wang Kun;Wu Xiangyang;Zhang Zhen
采用电化学安培法构建了一种竞争性免疫传感器，用于检测四溴双酚A（TBBPA）的重要衍生物四溴双酚A双（2-羟乙基）醚（TBBPA DHEE）。在该体系中，过氧化氢（H_2O_2）还原的电化学信号被放大，用安培法记录。同时，所合成的过氧化氢酶功能化金纳米粒子自组装聚合物纳米球对H2 O2具有良好的电催化性能，有利于增强电化学信号。在优化条件下，该方法的检出限为0.12 ng/mL，比传统的ELISA方法低7倍;准确度高，回收率为78-124%，相对标准偏差为2.1-8.3%，与ELISA方法一致;样品消耗量低（6 μL），成本低。将该方法应用于环境沃茨中TBBPA-DHEE的检测，结果表明该免疫传感器在水环境中痕量污染物的检测方面具有很大的潜力。
A competitive immunosensor was established using an electrochemical amperometric strategy for sensitive detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE), an important derivative of tetrabromobisphenol A (TBBPA). In this system, the amplified electrochemical signal towards the reduction of hydrogen peroxide (H2O2) was recorded by amperometric method. Meanwhile, the synthetized catalase functionalized AuNPs-loaded self-assembled polymer nanospheres showed an excellent electrocatalytic ability to catalyse H2O2, which was beneficial for strengthening the electrochemical signals. Under the optimized conditions, this method displayed: (i) low detection limits (0.12 ng/mL, 7 times lower than the traditional ELISA with the same antibody); (ii) satisfactory accuracy (recoveries, 78–124%; RSD, 2.1–8.3%) and good agreement with the corresponding ELISA; (iii) low sample consumption (6 μL) and low cost. The proposed approach was applied for investigation of TBBPA-DHEE from environmental waters, and our results indicated that this immunosensor has great potential to detect the trace pollutants in aquatic environments.