Differential microglial activation between acute stress and lipopolysaccharide treatment
Differential microglial activation between acute stress and lipopolysaccharide treatment
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DOI：
10.1016/j.jneuroim.2008.11.007
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发表时间：
2009-02-15
影响因子：
3.3
通讯作者：
Hashimoto, Makoto
中科院分区：
文献类型：
--
作者：
Sugama, Shuei;Takenouchi, Takato;Hashimoto, Makoto
急性应激被证明可以诱导包括中脑导水管周围灰质（PAG）在内的几个脑区的形态学小胶质细胞活化，该区域在对不可控制的应激、威胁、焦虑和疼痛的行为反应中起重要作用。为了确定神经元激活是否可能参与应激诱导的小胶质细胞激活，本研究调查了PAG中的神经元活性（以c-Fos表达测量）与形态学小胶质细胞激活之间的相关性。急性应激后，PAG中的小胶质细胞形态学活化和c-Fos表达增加，但周围中脑中没有。双重免疫组织化学和拓扑分析表明，小胶质细胞激活发生在邻近的反应神经元。相比之下，脂多糖（LPS）治疗诱导小胶质细胞活化，即使在PGA以及中脑的其余部分的神经元反应的情况下。这些发现表明，在应激过程中小胶质细胞活化的机制可能不同于感染或炎症。这也表明表达c-Fos蛋白的神经元细胞可能在触发小胶质细胞活化中起一定作用。(C)2008 Elsevier B. V.保留所有权利。
Acute stress was demonstrated to induce morphological microglial activation in several brain regions including the midbrain periaqueductal gray (PAG), an area that plays important roles in behavioral responses to uncontrollable stress, threat, anxiety, and pain. To determine whether neuronal activation may be involved in the stress-induced microglial activation, the present study investigated the correlation between neuronal activity measured as c-Fos expression and morphological microglial activation in the PAG. Acute stress was followed by morphological activation of microglia and increased c-Fos expression in the PAG but not in the surrounding midbrain. Double immunohistochemistry and topological analysis demonstrated that microglial activation occurred adjacent to responsive neurons. By contrast, lipopolysaccharide (LPS) treatment induced microglial activation even in the absence of neuronal responses in the PGA as well as in the rest of the midbrain. These findings suggest that the mechanism of microglial activation during stress may differ from those of infection or inflammation. It also indicates that the neuronal cells expressing c-Fos protein may play some roles to trigger microglial activation. (C) 2008 Elsevier B.V. All rights reserved.