Magnetically levitated mesenchymal stem cell spheroids cultured with a collagen gel maintain phenotype and quiescence.
Magnetically levitated mesenchymal stem cell spheroids cultured with a collagen gel maintain phenotype and quiescence.
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DOI：
10.1177/2041731417704428
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发表时间：
2017-01
影响因子：
8.2
通讯作者：
Berry CC
中科院分区：
文献类型：
--
作者：
Lewis NS;Lewis EE;Mullin M;Wheadon H;Dalby MJ;Berry CC
多细胞球体是一种成熟的三维细胞培养系统。球体通常是通过悬滴或非贴壁培养产生的；然而，一种新兴的技术是使用磁悬浮。在这里，间充质干细胞球体是使用磁性纳米颗粒产生的，随后在I型胶原凝胶中培养，以期开发一个骨髓利基环境。细胞被装载了磁性纳米颗粒，并悬浮在外部磁铁下，诱导多细胞球体自组装。球体中的细胞存活，与相应的单层对照相比，保持干细胞表型，并处于静止状态。有趣的是，与其他常用的球体系统相比，即使随着球体大小的增加，也没有观察到核心球体坏死。这种间充质干细胞球状培养提供了一个潜在的平台，在体外模拟骨髓干细胞的生态位，阐明细胞之间的相互作用，以及一个有用的模型，药物输送研究。
Multicellular spheroids are an established system for three-dimensional cell culture. Spheroids are typically generated using hanging drop or non-adherent culture; however, an emerging technique is to use magnetic levitation. Herein, mesenchymal stem cell spheroids were generated using magnetic nanoparticles and subsequently cultured within a type I collagen gel, with a view towards developing a bone marrow niche environment. Cells were loaded with magnetic nanoparticles, and suspended beneath an external magnet, inducing self-assembly of multicellular spheroids. Cells in spheroids were viable and compared to corresponding monolayer controls, maintained stem cell phenotype and were quiescent. Interestingly, core spheroid necrosis was not observed, even with increasing spheroid size, in contrast to other commonly used spheroid systems. This mesenchymal stem cell spheroid culture presents a potential platform for modelling in vitro bone marrow stem cell niches, elucidating interactions between cells, as well as a useful model for drug delivery studies.