Characterization of antisera to paired helical filaments and tau. Implication for the extent of tau tightly bound to paired helical filaments.
Characterization of antisera to paired helical filaments and tau. Implication for the extent of tau tightly bound to paired helical filaments.
复制标题
配对螺旋丝和 tau 蛋白抗血清的表征。
作者：
Y. Ihara;J. Kondo;R. Miura;Y. Nakagawa;H. Mori;T. Honda
对配对螺旋丝（PHF）和tau的两种抗血清进行了表征，这两种抗血清在组织切片上对神经原纤维缠结的染色相似。Anti-PHF只与tau蛋白的羧基末端反应，而anti - tau蛋白则与氨基末端反应。在用萨科齐（sarkozy -PHF）制备的PHF印迹中，anti-PHF从凝胶的顶部到底部广泛地染色，而anti- tau不染色这种涂片，而是标记少量与萨科齐-PHF结合的完整的tau。LDS或sds不溶性的萨科齐- phf残留物被标记为抗phf，但不标记为抗tau。抗tau蛋白定义的表位对蛋白酶敏感，而抗phf定义的表位则不受影响。基于这些观察，我们得出结论，萨科齐- phf主要由tau蛋白的羧基端区组成，并伴有少量完整的tau蛋白在其表面。
The two antisera to paired helical filaments (PHF) and tau, which stained neurofibrillary tangles similarly in tissue section, were characterized. Anti-PHF reacted exclusively with the carboxyl terminal portion of tau, whereas antitau reacted with the amino terminal portion. In the blots of PHF prepared in Sarkosyl (Sarkosyl-PHF), anti-PHF stained an extensive smear from the top to the bottom of the gel, while antitau did not stain this kind of smear but labeled a small amount of intact tau that was bound to Sarkosyl-PHF. An LDS- or SDS-insoluble residue of Sarkosyl-PHF was labeled with anti-PHF, but not with antitau. Antitau-defined epitopes were susceptible to protease, whereas anti-PHF-defined epitopes were unaffected. Based on these observations, we conclude that Sarkosyl-PHF were composed largely of the carboxyl terminal region of tau and accompanied by a small amount of intact tau on their surfaces.