Lotus-leaf-like structured heparin-conjugated poly(L-lactide-co-epsilon-caprolactone) as a blood compatible material.
Lotus-leaf-like structured heparin-conjugated poly(L-lactide-co-epsilon-caprolactone) as a blood compatible material.
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DOI：
10.1016/j.colsurfb.2012.11.016
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发表时间：
2013-03
期刊：
影响因子：
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通讯作者：
J. Lim;S. Kim;Soo-Hyun Kim
中科院分区：
文献类型：
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作者：
J. Lim;S. Kim;Soo-Hyun Kim
通过将肝素直接偶联到聚(L-丙交酯-co-ɛ-己内酯)(PLCL)上，合成了一种肝素偶联生物降解聚合物，并将其制成荷叶状结构薄膜。探讨荷叶状结构肝素结合物(LHPlcL)在血管组织工程中的应用。用扫描电子显微镜(SEM)和接触角测试仪研究了膜的表面结构在疏水性、莲花效应以及在人体全血中的抗血栓作用。使用拉伸强度测试机进行恢复测试，并使用甲苯胺蓝比色法对结合肝素进行定量分析。共轭肝素的浓度为0.14μg/mgH-plcL，与荷叶状表面的接触角约为120°。此外，与未经处理的PLCL膜相比，LH-PLCL膜在全血中产生的血小板粘附率更低(约1.4%)。这些结果表明了结合肝素的独特性质和荷叶状结构。这种新型的黄体生成素-PLCL聚合物可作为植入式医疗器械和组织工程的血液/组织相容性生物降解材料。
A heparin-conjugated biodegradable polymer was synthesized by direct coupling of heparin to poly(L-lactide-co-ɛ-caprolactone) (PLCL) and was manufactured into lotus-leaf-like structured films. We evaluated whether lotus-leaf-like structured heparin-conjugated PLCL (LH-PLCL) could be applied to blood vessel tissue engineering. Differences in the surface structures of the films with respect to hydrophobicity and the lotus effect as well as the antithrombotic efficiency in human whole blood were examined using scanning electron microscopy (SEM) and a contact angle meter. Recovery testing was conducted using a tensile strength testing machine, and quantitative analysis of conjugated heparin was performed using the toluidine blue colorimetric method. The concentration of conjugated heparin was 0.14μg/mg H-PLCL, and the contact angle with the lotus-leaf-like surface was approximately 120°. Furthermore, the LH-PLCL film yielded a lower platelet adhesion rate (around less than 1.4%) in whole blood than that yielded by an untreated PLCL film. These results indicate a unique property of bound heparin and the lotus-leaf-like structure. This novel LH-PLCL polymer could be applied as a blood/tissue compatible biodegradable material for implantable medical devices and tissue engineering.