A rice (Oryza sativa L.) MAP kinase gene, OsMAPK44, is involved in response to abiotic stresses
A rice (Oryza sativa L.) MAP kinase gene, OsMAPK44, is involved in response to abiotic stresses
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DOI：
10.1007/s11240-005-9064-0
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发表时间：
2006-05-01
影响因子：
3
通讯作者：
Park, SC
中科院分区：
文献类型：
--
作者：
Jeong, MJ;Lee, SK;Park, SC
我们已经分离并鉴定了一个推定的水稻MAPK基因（命名为OsMAPK 44），其编码具有MAPK家族特征和磷酸化激活基序TDY的593个氨基酸的蛋白质。OsMAPK44的氨基酸序列与其它水稻MAPKs的氨基酸序列同源性较高。正常情况下，OsMAPK44基因在粳稻（O. sativa L. Donggin）。该基因的mRNA表达受盐和干旱处理的高度诱导，但不受冷处理。此外，外源ABA和H2O2可上调OsMAPK44的mRNA水平。当我们比较盐敏感籼稻品种（IR64）和盐抗性籼稻品种（Pokkali）之间的OsMAPK44基因表达水平时，它们在盐处理下的表达动力学表现出一些差异。OsMAPK44基因在Pokkali中的表达在30 min内略有上调，然后迅速消失，而IR64在下调后1 h仍保持表达。在盐胁迫下，0sMAPK44过表达转基因水稻植株的伤害程度低于0sMAPK44抑制转基因植株，钾钠比值高于0sMAPK44抑制转基因植株，表明0sMAPK44可能通过在盐胁迫下维持有利的离子平衡而起到防止伤害的作用。
We have isolated and characterized a putative rice MAPK gene (designated OsMAPK44) encoding for a protein of 593 amino acids that has the MAPK family signature and phosphorylation activation motif, TDY. Alignment of the predicted amino acid sequences of OsMAPK44 showed high homology with other rice MAPKs. Under normal conditions, the OsMAPK44 gene is highly expressed in root tissues, but relatively less in leaf and stem tissues of the japonica type rice plant (O. sativa L. Donggin). mRNA expression of the gene is highly inducible by salt and drought treatment, but not by cold treatment. Moreover, the mRNA level of the OsMAPK44 is up-regulated by exogenously applied Abscisic acid (ABA) and H2O2. When we compared the OsMAPK44 gene expression level between a salt sensitive indica cultivar (IR64) and a salt resistant indica cultivar (Pokkali), they showed some difference in expression kinetics with the salt treatment. OsMAPK44 gene expression in Pokkali was slightly up-regulated within 30 min and then disappeared rapidly, while IR64 maintained its expression for 1 h following down-regulation. Under the salinity stress, OsMAPK44 overexpression transgenic rice plants showed less damage and greater ratio of potassium and sodium than OsMAPK44 suppressed transgenic lines did, suggesting that OsMAPK44 may have a role to prevent damages due to working for favorable ion balance in the presence of salinity.