Patterns of Gene Expression in the Bovine Corpus Luteum Following Repeated Intrauterine Infusions of Low Doses of Prostaglandin F2alpha
Patterns of Gene Expression in the Bovine Corpus Luteum Following Repeated Intrauterine Infusions of Low Doses of Prostaglandin F2alpha
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DOI：
10.1095/biolreprod.111.094870
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发表时间：
2012-04-01
影响因子：
3.6
通讯作者：
Wiltbank, Milo C.
中科院分区：
文献类型：
--
作者：
Atli, Mehmet O.;Bender, Robb W.;Wiltbank, Milo C.
自然黄体溶解涉及由非妊娠子宫释放的前列腺素F2 α（PGF）的多个脉冲。本研究调查了来自五个不同途径的18个基因的表达，在多次低剂量PGF脉冲后。发情周期第9天的奶牛每隔6小时接受4次0.25 ml磷酸盐缓冲盐水（PBS）或PGF（0.5 mg PGF溶于0.25 ml PBS中）宫内输注。在每次PBS或PGF输注后30分钟收集黄体活检样品。有四个治疗组：对照组（n = 5; 4次PBS输注）、4XPGF组（4次PGF输注; n = 5）、2XPGF-非消退组（2次PGF输注; n = 5; PGF-PBS-PGF-PBS;治疗后无消退）和2XPGF-消退组（PGF-PBS-PGF-PBS;治疗后消退; n = 5）。正如预期的，第一次PGF脉冲增加了即刻早期基因JUN、FOS、NR 4A 1和EGFR 1的mRNA，但出乎意料地也增加了类固醇生成（星星）和血管生成（VEGFA）途径的mRNA。第二次PGF脉冲诱导立即早期基因和与免疫系统激活相关的基因（IL 1B、FAS、FASLG、IL 8）。然而，VEGFA和星星的mRNA被第二次PGF输注降低。在第三和第四PGF脉冲后，明显的基因表达的黄体溶解模式是明显的，抑制类固醇生成和血管生成途径，而诱导免疫系统激活和PGF产生的途径。PGF诱导的基因表达模式在第一次PGF脉冲后不用于黄体溶解的黄体中相似（2X-非回归），但在第二次PGF脉冲后非常明显。因此，尽管最初的PGF脉冲诱导了许多途径的mRNA，但第二次和以后的PGF脉冲似乎已经设定了导致黄体溶解的基因表达的独特模式。
Natural luteolysis involves multiple pulses of prostaglandin F2alpha (PGF) released by the nonpregnant uterus. This study investigated expression of 18 genes from five distinct pathways, following multiple low-dose pulses of PGF. Cows on Day 9 of the estrous cycle received four intrauterine infusions of 0.25 ml of phosphate-buffered saline (PBS) or PGF (0.5 mg of PGF in 0.25 ml of PBS) at 6-h intervals. A luteal biopsy sample was collected 30 min after each PBS or PGF infusion. There were four treatment groups: Control (n = 5; 4 PBS infusions), 4XPGF (4 PGF infusions; n = 5), 2XPGF-non-regressed (2 PGF infusions; n = 5; PGF-PBS-PGF-PBS; no regression after treatments), and 2XPGF-regressed (PGF-PBS-PGF-PBS; regression after treatments; n = 5). As expected, the first PGF pulse increased mRNA for the immediate early genes JUN, FOS, NR4A1, and EGR1 but unexpectedly also increased mRNA for steroidogenic (STAR) and angiogenic (VEGFA) pathways. The second PGF pulse induced immediate early genes and genes related to immune system activation (IL1B, FAS, FASLG, IL8). However, mRNA for VEGFA and STAR were decreased by the second PGF infusion. After the third and fourth PGF pulses, a distinctly luteolytic pattern of gene expression was evident, with inhibition of steroidogenic and angiogenic pathways, whereas, there was induction of pathways for immune system activation and production of PGF. The pattern of PGF-induced gene expression was similar in corpus luteum not destined for luteolysis (2X-non-regressed) after the first PGF pulse but was very distinct after the second PGF pulse. Thus, although the initial PGF pulse induced mRNA for many pathways, the second and later pulses of PGF appear to have set the distinct pattern of gene expression that result in luteolysis.