NONFIBRILLAR BETA-AMYLOID PROTEIN IS ASSOCIATED WITH SMOOTH-MUSCLE CELLS OF VESSEL WALLS IN ALZHEIMER-DISEASE
NONFIBRILLAR BETA-AMYLOID PROTEIN IS ASSOCIATED WITH SMOOTH-MUSCLE CELLS OF VESSEL WALLS IN ALZHEIMER-DISEASE
复制标题
DOI：
10.1097/00005072-199411000-00011
复制
发表时间：
1994-11-01
影响因子：
3.2
通讯作者：
WISNIEWSKI, HM
中科院分区：
文献类型：
--
作者：
FRACKOWIAK, J;ZOLTOWSKA, A;WISNIEWSKI, HM
研究人员对阿尔茨海默病 (AD) 的脑膜血管和死后 16 小时内尸检获得的对照脑标本进行了研究。系列切片用硫黄素 S 和刚果红染色，并针对 β-淀粉样前体蛋白 (β PP) 和 β-蛋白以及平滑肌特异性蛋白肌球蛋白、α-肌动蛋白和结蛋白的存在进行免疫染色。通过免疫印迹研究分离的血管中是否存在 β PP、β PP 片段和 β 蛋白。血管壁各层β蛋白呈强免疫阳性的动脉在硫黄素S和刚果红染色中淀粉样原纤维也呈阳性。在受影响较小的血管中，β-蛋白的局灶性免疫染色位于平滑肌细胞细胞质中的中膜或肌细胞之间形成的颗粒中。细胞质β蛋白和细胞间存在的一些小沉积物对淀粉样原纤维呈阴性。从含有β-蛋白免疫反应性材料的样本中分离的血管含有3kD、4.2-4.5kD、8.5-9kD和17.5kD β-蛋白免疫反应性条带。在通过免疫细胞化学评估为 β 蛋白阴性的样本中未发现这些条带。这些数据表明，在AD血管壁中淀粉样蛋白形成的过程中，非纤维状、单体和寡聚β-蛋白积累。
Meningeal blood vessels were studied in Alzheimer disease (AD) and control brain specimens obtained from autopsies within 16 hours after death. Serial sections were stained with thioflavine S and Congo red and immunostained for the presence of beta-amyloid precursor protein (beta PP) and beta-protein and for smooth muscle-specific proteins myosin, alpha-actin, and desmin. Isolated blood vessels were studied by immunoblotting for the presence of beta PP, fragments of beta PP, and beta-protein. The arteries that were strongly immunopositive for beta-protein in all layers of the walls were also positive for amyloid fibrils on thioflavine S and Congo red stainings. The focal immunostaining for beta-protein in less affected vessels was located in the tunica media in the cytoplasm of smooth muscle cells or formed granules between myocytes. The cytoplasmic beta-protein and some of the small deposits present between cells were negative for amyloid fibrils. The Vessels isolated from specimens containing beta-protein-immunoreactive material contained 3 kD, 4.2-4.5 kD, 8.5-9 kD, and 17.5 kD beta-protein-immunoreactive bands. These bands were not found in the samples assessed as beta-protein-negative by immunocytochemistry. These data indicate that during formation of amyloid in AD vessel walls, nonfibrillar, monomeric, and oligomeric beta-protein accumulate.