The MORPHEUS II protein crystallization screen.
The MORPHEUS II protein crystallization screen.
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DOI：
10.1107/s2053230x1500967x
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发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Gorrec F
中科院分区：
文献类型：
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作者：
Gorrec F
MORPHEUS II是一种96条件的初始结晶筛重新制定。该屏幕结合了从蛋白质数据库中选择的试剂，以产生在传统条件下无法观察到的晶体。此外，该配方有利于晶体的优化和低温保护。高质量的大分子晶体是x射线衍射测定蛋白质结构的先决条件。不幸的是，从结晶实验中得到的衍射质量晶体的相对产率往往很低。在此背景下，创新的结晶筛网配方不断被开发出来。在过去，MORPHEUS是一种筛管，在每种情况下都集成了从蛋白质数据库中选择的添加剂、冷冻保护剂和缓冲系统的混合物。在这里，MORPHEUS II，一个后续的原始96条件初始屏幕，被描述。当在传统的初始筛选中没有观察到晶体时，选择试剂以产生晶体。此外，筛网还包括用于实验相的重原子和小多元醇，以确保晶体的低温保护。由此产生的新条件的适用性通过多种蛋白质样品的结晶显示，并将其效率与商业上可用的条件进行比较。
MORPHEUS II is a 96-condition initial crystallization screen formulated de novo. The screen incorporates reagents selected from the Protein Data Bank to yield crystals that are not observed in traditional conditions. In addition, the formulation facilitates the optimization and cryoprotection of crystals. High-quality macromolecular crystals are a prerequisite for the process of protein structure determination by X-ray diffraction. Unfortunately, the relative yield of diffraction-quality crystals from crystallization experiments is often very low. In this context, innovative crystallization screen formulations are continuously being developed. In the past, MORPHEUS, a screen in which each condition integrates a mix of additives selected from the Protein Data Bank, a cryoprotectant and a buffer system, was developed. Here, MORPHEUS II, a follow-up to the original 96-condition initial screen, is described. Reagents were selected to yield crystals when none might be observed in traditional initial screens. Besides, the screen includes heavy atoms for experimental phasing and small polyols to ensure the cryoprotection of crystals. The suitability of the resulting novel conditions is shown by the crystallization of a broad variety of protein samples and their efficiency is compared with commercially available conditions.