Effect of cytokine-encoding plasmid delivery on immune response to Japanese encephalitis virus DNA vaccine in mice
Effect of cytokine-encoding plasmid delivery on immune response to Japanese encephalitis virus DNA vaccine in mice
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DOI：
10.1111/j.1348-0421.2005.tb03739.x
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发表时间：
2005-01-01
影响因子：
2.6
通讯作者：
Vrati, S
中科院分区：
文献类型：
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作者：
Bharati, K;Appaiahgari, MB;Vrati, S
我们先前已经证明，用合成日本脑炎病毒（JEV）包膜蛋白的质粒pMEa免疫小鼠诱导抗JEV的免疫应答和细胞免疫应答。我们现在表明，在pMEa给药后4天，将pMEa和pGM-CSF（编码鼠粒细胞-巨噬细胞集落刺激因子或pIL-2，编码鼠白细胞介素-2）肌肉内联合给药小鼠，增强了抗JEV抗体滴度。这并没有提高免疫小鼠对JEV的保护水平。然而，使用基因枪在小鼠中皮内共施用pMEa和pGM-CSF增强了抗JEV抗体滴度，导致小鼠中针对致死性JEV攻击的保护水平增加。
We have previously shown that immunization of mice with plasmid pMEa synthesizing Japanese encephalitis virus (JEV) envelope protein induced anti-JEV Immoral and cellular immune responses. We now show that intra-muscular co-administration of mice with pMEa and pGM-CSF, encoding murine granulocyte-macrophage colony-stimulating factor or pIL-2, encoding murine interleukin-2 given 4 days after pMEa, augmented anti-JEV antibody titers. This did not enhance the level of protection in immunized mice against JEV. However, intra-dermal co-administration of pMEa and pGM-CSF in mice using the gene gun, enhanced anti-JEV antibody titers resulting in an increased level of protection in mice against lethal JEV challenge.