Non-metastasising variants selected from metastasising melanoma cells
Non-metastasising variants selected from metastasising melanoma cells
复制标题
选自转移性黑色素瘤细胞的非转移性变体
作者：
T. Tao;M. Burger
细胞表面可能参与癌细胞的转移过程1。为了确定膜特性是否影响或反映转移能力，必须具有相同肿瘤的转移性和非转移性变体。与表面碳水化合物特异性结合的凝集素2已成功地用作选择剂，以获得具有膜碳水化合物改变的细胞3 -5。我们已经选择了黑色素瘤细胞的变异体，在使用毒性浓度的凝集素的表面差异，希望这些变异体将表现出不同的转移能力。我们在这里报告的变异分离转移性黑色素瘤细胞耐毒性浓度的麦胚凝集素（WGA）。这些变体显示减少至转移能力的丧失和改变的表面性质。
THE cell surface may be involved in the metastatic process of cancer cells1. To determine whether membrane properties influence or reflect the metastasising capacity, it is essential to have metastasising and non-metastasising variants of the same tumour. Lectins which bind specifically to surface carbohydrates2 have been used successfully as selective agents to obtain cells with membrane carbohydrate alterations3–5. We have selected variants of melanoma cells for surface differences in using toxic concentrations of lectins in the hope that such variants would exhibit different metastasising capacities. We report here the isolation of variants from metastasising melanoma cells resistant to toxic concentrations of wheat germ agglutinin (WGA). These variants show reduced to a loss of metastasising capacity and altered surface properties.