A chamber and artificial dura method for long-term optical imaging in the monkey
A chamber and artificial dura method for long-term optical imaging in the monkey
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DOI：
10.1016/s0165-0270(01)00475-7
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发表时间：
2002-01-15
影响因子：
3
通讯作者：
Roe, AW
中科院分区：
文献类型：
--
作者：
Chen, LM;Heider, B;Roe, AW
在非人类灵长类动物中长时间的光学成像提出了严重的挑战，因为必须去除硬脑膜以暴露皮质表面。我们提出了一种新型的尼龙成像室与透明的人工硬脑膜植入物，它允许重复的，长期的光学记录从皮层。将腔室的圆柱体插入颅骨环钻中，并用最少的螺钉和丙烯酸水泥固定到位。一个圆形的人工硬脑膜补片与垂直壁保护皮质表面和减缓腔室内硬脑膜组织的再生长。一个由与气缸相同的材料制成的盖被拧入腔室并完全密封。在1-4个月的时间内，腔室需要最低限度的维护，并且在没有局部抗生素应用的情况下保持无感染。我们在同一动物中重复进行光学成像，具有缩短准备时间的优点。为了允许从不同成像会话获得的地图的精确对齐和比较，我们开发了一个程序，计算不同放大率，平移和扭曲的地图之间的二维空间变换。我们认为，这些方法提供了一个实用的解决方案，长期的光学成像在麻醉或警觉的猴子。非金属材料的独家使用提供了更轻和更紧凑的植入物的好处，以及在腔室植入后进行MRI扫描的可能性。(C)2002 Elsevier Science B. V.保留所有权利。
Optical imaging over extended periods of time in non-human primates presents serious challenges because the dura mater must be removed to expose the cortical surface. We present a novel nylon imaging chamber with a transparent artificial dura implant, which allows repeated, long-term optical recordings from the cortex. The cylinder of the chamber is inserted into a cranial trephination and held in place with a minimum of screws and acrylic cement. A round patch of artificial dura with a perpendicular wall protects the cortical surface and slows re-growth of dural tissue within the chamber. A cap, manufactured from the same material as the cylinder, is screwed into the chamber and seals it completely. Over a period of 1-4 months, the chamber required a minimum of maintenance and stayed infection-free without local antibiotic application. We repeatedly performed optical imaging in the same animal with the advantages of shortened preparation time. To permit precise alignment and comparison of maps obtained from different imaging sessions, we developed a program that calculated a 2-dimensional spatial transformation between maps of different magnifications, translations, and distortions. We suggest that these methods provide a practical solution to long-term optical imaging in the anesthetized or alert monkey. The exclusive use of non-metallic materials offers the benefit of a lighter and more compact implant, and the possibility to perform MRI scans after chamber implantation. (C) 2002 Elsevier Science B.V. All rights reserved.