Anucleolate frog embryos contain ribosomal DNA sequences and a nucleolar antigen.
Anucleolate frog embryos contain ribosomal DNA sequences and a nucleolar antigen.
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无核青蛙胚胎含有核糖体 DNA 序列和核仁抗原。
DOI：
10.1016/0012-1606(84)90205-7
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发表时间：
1984
影响因子：
2.7
通讯作者：
Reeder,RH
中科院分区：
文献类型：
--
作者：
Steele,RE;Thomas,PS;Reeder,RH
先前对非洲爪蟾胚胎纯合核仁缺失突变的研究已经得出结论，这些胚胎含有很少的，如果有的话，18s和28s核糖体rna基因拷贝。使用杂交限制DNA的内切酶酶切发现，事实上，少量的核糖体DNA仍然存在于这样的胚胎中。这些胚胎中的核糖体DNA似乎包括一些正常的重复序列和各种不同寻常的片段，这些片段含有间隔序列或基因序列。在硬皮病患者血清中发现的抗体与野生型胚胎核仁中的抗原发生反应。在无核胚中，这种抗原存在于所谓的假核仁和许多细胞核内的小体中。
Previous studies ofXenopus laevisembryos homozygous for the nucleolar deletion mutation have concluded that these embryos contain few, if any, copies of the genes for the 18 S and 28 S ribosomal RNAs. Using hybridization to restriction endonuclease digests of DNA it is found, in fact, that a small amount of ribosomal DNA is still present in such embryos. The ribosomal DNA in these embryos appears to include a few normal repeats together with a variety of unusual fragments containing either spacer or gene sequences. An antibody found in the serum of a scleroderma patient reacts with an antigen localized in the nucleoli of wild-type embryos. In anucleolate embryos this antigen is found in the so-called pseudonucleoli and in many small bodies in the nuclei.