Edwardsiella piscicida Ail1: An outer membrane protein required for host infection
Edwardsiella piscicida Ail1: An outer membrane protein required for host infection
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DOI：
10.1016/j.aquaculture.2023.739289
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发表时间：
2023-01-24
期刊：
影响因子：
4.5
通讯作者：
Sun，Yuan-yuan
中科院分区：
文献类型：
--
作者：
Li，Mo-fei;Du，Yu-ting;Sun，Yuan-yuan
杀爱德华氏菌（Edwardsiellapiscicidae）是一种重要的病原微生物，可引起多种宿主的系统性感染，包括硬骨鱼、两栖动物和哺乳动物。Ail是参与毒力的表面蛋白。然而，在E.杀鱼的本研究检测了弗罗姆杆菌Ail分子的功能和免疫保护特性。Ail 1具有保守的Ail/Lom超家族结构域，并暴露于细菌表面。ofail 1突变显著降低了受感染鱼在酸性和氧化环境中的存活率、运动性、生物膜形成、细胞感染、组织传播和死亡。TX 01 Δ ail 1基因的过表达可挽救TX 01 Δ ail 1的丧失能力。重组Ail 1（rAil 1）能与牙鲆外周血白细胞相互作用，影响E.杀鱼素体外转染。疫苗试验表明，rAil 1可作为一种潜在的亚单位疫苗，诱导出有效的抗E.杀鱼感染rAil 1免疫的鱼血清中产生了针对rAil 1的特异性抗体，增强了血清补体的杀菌活性。此外，rAil 1免疫的鱼显示出更高的ROS和巨噬细胞的酸性磷酸酶活性。以上结果表明，Ail 1在大肠杆菌的毒力中起着关键作用。杀鱼素可作为水产养殖的亚单位疫苗。
Edwardsiellapiscicidais a significant pathogenic microorganism, and can lead to systematic infections in multifold hosts, including teleost, amphibians, and mammals. Ail is a surface protein involved in virulence. However, the underlining mechanism is still to be investigated inE. piscicida. This study examined the functions and immunoprotective properties of an Ail molecule fromE. piscicida.Ail1 possessed a conserved Ail/Lom superfamily domain and was exposed to the bacterial surface. Mutation ofail1significantly reduced the survival rate under acid and oxidizing environment, motility, biofilm formation, cellular infection, tissue dissemination, and causing mortality in infected fish. The loss abilities of TX01Δail1were rescued by overexpression of theail1gene. Recombinant Ail1 (rAil1) could interact with peripheral blood leukocytes from Japanese flounder and affectE. piscicidainfectionin vitro. Vaccine trial showed that rAil1 was used as a potential subunit vaccine and could induce effective immune protection againstE. piscicidainfection. Fish vaccinated with rAil1 produced the specific antibody against rAil1 in serum, which increased the bactericidal activity of serum complement. In addition, rAil1-vaccinated fish displayed the higher ROS and acid phosphatase activity of macrophages. The above findings indicated that Ail1 played a pivotal role in the virulence ofE. piscicidaand may be explored as a subunit vaccine in aquaculture.