A chlorate-resistant mutant defective in the regulation of nitrate reductase gene expression in arabidopsis defines a new HY locus
A chlorate-resistant mutant defective in the regulation of nitrate reductase gene expression in arabidopsis defines a new HY locus
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DOI：
10.1105/tpc.9.1.21
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发表时间：
1997-01-01
期刊：
影响因子：
11.6
通讯作者：
Cheng, CL
中科院分区：
文献类型：
--
作者：
Lin, Y;Cheng, CL
光既直接作为信号，又通过光合作用间接调节硝酸还原酶(NR)基因的表达。在这里，我们报告了一个新的抗氯酸盐突变体的分离和鉴定，该突变体在调节NR基因表达方面存在缺陷。在这个命名为cr88的拟南芥突变体中，NR2，而不是NR1或编码亚硝酸盐还原酶(NIR)的基因对光信号的反应受到损害。除NR2外，该突变体还损害了编码叶绿素白蛋白结合蛋白(CAB)和核酮糖二磷酸羧基酶(RBCs)小亚基的基因的光调节。这些结果表明，光调控NR2、CAB和RBC基因表达的途径与调控NR1和NIR基因表达的途径不同。对不同光谱下的脱黄化过程的研究表明，cr88在红光介导的脱黄化过程中存在缺陷。与不同的长下胚轴(Hy)突变体的互补试验表明，CR88发现了一个新的hy基因座。讨论了CR88的可能功能。
Light acts both directly as a signal and indirectly through photosynthesis to regulate the expression of genes encoding nitrate reductase (NR). Here, we report the isolation and characterization of a novel chlorate-resistant mutant that is defective in the regulation of NR gene expression. The response of NR2, but not NR1 or the gene encoding nitrite reductase (NIR), to light signals was impaired in this Arabidopsis mutant, designated cr88. In addition to NR2, the light regulation of the genes encoding the chlorophyll alb binding protein (CAB) and the small subunit of ribulose bisphosphate carboxylase (RBCS) was also impaired in this mutant. These results suggest that the pathway through which light regulates the expression of NR2, CAB, and RBCS genes is different from those that regulate the expression of NR1 and NiR. An examination of the deetiolation process under different light spectra showed that cr88 is defective in red light-mediated deetiolation. Complementation tests with various long hypocotyl (hy) mutants indicated that CR88 identifies a new HY locus. The possible functions of CR88 are discussed.