Parasporin, a human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis
Parasporin, a human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis
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DOI：
10.1128/cdli.7.4.625-634.2000
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发表时间：
2000-07-01
期刊：
影响因子：
--
通讯作者：
Ohba, M
中科院分区：
文献类型：
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作者：
Mizuki, E;Park, YS;Ohba, M
一个不寻常的性质，人类白血病细胞识别活性，与非杀虫苏云金芽孢杆菌土壤分离物的副孢子包涵体相关，并克隆了一个蛋白质（在本研究中命名为副孢子蛋白）负责的活性。该副孢蛋白由一个长2169 bp的基因编码，是一个由723个氨基酸残基组成的多肽，预计分子量为81,045。副孢蛋白序列包含苏云金芽孢杆菌Cry蛋白中常见的5个保守片段；然而，只有非常低的同源性(
An unusual property, human leukemic cell-recognizing activity, associated with parasporal inclusions of a noninsecticidal Bacillus thuringiensis soil isolate was investigated, and a protein (named parasporin in this study) responsible for the activity was cloned. The parasporin, encoded by a gene 2,169 bp long, was a polypeptide of 723 amino acid residues with a predicted molecular weight of 81,045. The sequence of parasporin contained the five conserved blocks commonly found in B. thuringiensis Cry proteins; however, only very low homologies (