ARGININE DEGRADATION IN PSEUDOMONAS-AERUGINOSA MUTANTS BLOCKED IN 2 ARGININE CATABOLIC PATHWAYS
ARGININE DEGRADATION IN PSEUDOMONAS-AERUGINOSA MUTANTS BLOCKED IN 2 ARGININE CATABOLIC PATHWAYS
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DOI：
10.1007/bf00382081
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
MERCENIER, A
中科院分区：
文献类型：
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作者：
HAAS, D;MATSUMOTO, H;MERCENIER, A
从铜绿假单胞菌中分离出胍丁胺利用缺陷突变株。AGUA和AUB的编码基因在染色体的30min区域定位于GPU和Ser-3之间，具有98%的共转导性。构建的Agu-Arc双突变体(受阻于精氨酸脱羧酶和精氨酸脱亚胺酶途径)有效地利用精氨酸作为唯一的碳源和氮源。这表明铜绿假单胞菌中存在进一步的精氨酸分解代谢途径。本研究的作图数据证实，在铜绿假单胞菌中，具有分解代谢功能的染色体基因并不像恶臭假单胞菌那样表现出超腹膜聚集性。
P. aeruginosa mutants defective in agmatine utilization (agu) were isolated. The genes encoding agmatine deiminase (aguA) and N-carbamoylputrescine amidinohydrolase (aguB) were 98% cotransducible and mapped between gpu and ser-3 in the 30 min region of the chromosome. Constructed agu arc double mutants (blocked in the arginine decarboxylase and arginine deiminase pathways) used arginine efficiently as the sole C and N source. This suggests the existence of a further arginine catabolic pathway in P. aeruginosa. The mapping data of this study confirm that in P. aeruginosa the chromosomal genes with catabolic functions do not show supraoperonic clustering as found in P. putida.