Ratio of remnant-like particle-cholesterol to serum total triglycerides is an effective alternative to ultracentrifugal and electrophoretic methods in the diagnosis of familial type III hyperlipoproteinemia.
Ratio of remnant-like particle-cholesterol to serum total triglycerides is an effective alternative to ultracentrifugal and electrophoretic methods in the diagnosis of familial type III hyperlipoproteinemia.
复制标题
残余颗粒胆固醇与血清总甘油三酯的比率是诊断家族性 III 型高脂蛋白血症时超速离心和电泳方法的有效替代方法。
DOI：
10.1093/clinchem/45.11.1981
复制
发表时间：
1999
影响因子：
9.3
通讯作者：
R. Havel
中科院分区：
文献类型：
--
作者：
Tao Wang;K. Nakajima;E. Leary;G. Warnick;J. Cohn;P. Hopkins;Lily L. Wu;D. Cilla;J. Zhong;R. Havel
背景
家族性III型高脂蛋白血症（HLP）的特征是存在β-迁移性VLDL（β-VLDL）和心血管疾病风险增加。通过测量VLDL-胆固醇（VLDL-C）与总血浆甘油三酯（TG）的比值或通过检测总VLDL中的β-VLDL来评估血浆β-VLDL。本研究的目的是比较残留样颗粒胆固醇（RLP-C）与总TG的比值与目前诊断III型HLP的方法的临床实用性。
方法
通过VLDL电泳检测β-VLDL用于定义III型HLP。研究了28例III型HLP患者和43例缺乏β-VLDL的受试者。所有受试者的空腹TG浓度均>2.26 mmol/L。受试者分为三组：第1组，血清总胆固醇</=5.18 mmol/L（n = 11）;第2组，总胆固醇>5.18 mmol/L且TG在2.26和9.04 mmol/L之间（n = 51）;第3组，TG>9.04 mmol/L（n = 9）。
结果
在第2组中，RLP-C与总TG摩尔比>/=0.23（使用mg/dL时>/=0.10）和VLDL-C与总TG摩尔比>/=0.69（使用mg/dL时>/=0.30）分别正确分类了94%和90%的受试者。RLP-C与总TG比值在诊断III型HLP中的实用性在其他两组患者中降低。
结论
当用于适当的目标人群时，RLP-C与总TG的比值是诊断III型HLP的超离心和电泳方法的方便和有效的替代方法。
BACKGROUND
Familial type III hyperlipoproteinemia (HLP) is characterized by the presence of beta-migrating VLDL (beta-VLDL) and increased risk of cardiovascular disease. Assessment of plasma beta-VLDL is achieved by measuring the ratio of VLDL-cholesterol (VLDL-C) to total plasma triglycerides (TGs) or by detecting beta-VLDL in total VLDL. The objective of this study was to compare the clinical utility of the ratio of remnant-like particle-cholesterol (RLP-C) to total TGs with that of the current methods for diagnosing type III HLP.
METHODS
Detection of beta-VLDL by electrophoresis of VLDL was used to define type III HLP. Twenty-eight patients with type III HLP and 43 subjects lacking beta-VLDL were investigated. Fasting TG concentrations were >2.26 mmol/L in all subjects. Subjects were separated into three groups: group 1, serum total cholesterol </=5.18 mmol/L (n = 11); group 2, total cholesterol >5.18 mmol/L and TGs between 2.26 and 9.04 mmol/L (n = 51); and group 3, TGs >9.04 mmol/L (n = 9).
RESULTS
In group 2, a RLP-C-to-total TG molar ratio >/=0.23 (>/=0.10 when using mg/dL) and a VLDL-C-to-total TG molar ratio >/=0.69 (>/=0.30 when using mg/dL) correctly classified 94% and 90% of the subjects, respectively. The utility of the RLP-C-to-total TG ratio in diagnosing type III HLP decreased in patients in the other two groups.
CONCLUSION
When used in an appropriate target population, the RLP-C-to-total TG ratio is a convenient and effective alternative to ultracentrifugal and electrophoretic methods for diagnosing type III HLP.
影响因子：
6.5
作者：
J. Hixson;D. T. Vernier
通讯作者：
J. Hixson;D. T. Vernier
使用载脂蛋白 B-100 单克隆抗体从人血浆中分离出富含甘油三酯的脂蛋白的载脂蛋白 E 富集组分的特性。
影响因子：
6.5
作者：
Campos,E;Nakajima,K;Tanaka,A;Havel,RJ
通讯作者：
Havel,RJ
残余脂蛋白胆固醇和甘油三酯参考范围来自弗雷明汉心脏研究。
影响因子：
9.3
作者：
McNamara,JR;Shah,PK;Nakajima,K;Cupples,LA;Wilson,PW;Ordovas,JM;Schaefer,EJ
通讯作者：
Schaefer,EJ