Encapsulation studies and selective membrane permeability properties of self-assembly hollow nanospheres
Encapsulation studies and selective membrane permeability properties of self-assembly hollow nanospheres
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DOI：
10.1039/c0sm00896f
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发表时间：
2011-01
期刊：
影响因子：
3.4
通讯作者：
Wei Ha;Xianwei Meng;Q. Li;Minmin Fan;S. Peng;L. Ding;X. Tian;S. Zhang;Bang‐Jing Li
中科院分区：
文献类型：
--
作者：
Wei Ha;Xianwei Meng;Q. Li;Minmin Fan;S. Peng;L. Ding;X. Tian;S. Zhang;Bang‐Jing Li
提出了一种用于酶包封的自组装中空纳米球。通过棒状线圈Alg-g-PEG和α-CDs配合物的自组装，将酶分子直接封装在水溶液中的空心球体中。这些al -g- peg /α-CD空心球具有半渗透性，可以阻止大的酶分子离开，同时允许小的底物和产物通过，以维持酶的活性。通过包封不同探针，研究了Alg-g-PEG/α-CD空心纳米球的渗透性能。结果表明，空心纳米球的摩尔质量截止值（MMCO）在20 ~ 40 kDa之间。对al -g- peg /α-CD空心纳米球的包封能力进行了研究，结果表明其包封l -天冬酰胺酶的能力达到最大值。此外，l -天冬酰胺酶的包封行为表现出PEG接枝密度依赖性。
This paper presents a kind of self-assembled hollow nanosphere for enzyme encapsulation. The enzyme molecules were encapsulated in hollow spheres directly in aqueous solution through the self-assembly of rod-coil Alg-g-PEG and α-CDs complexes. These Alg-g-PEG/α-CD hollow spheres showed semi-permeability which could prevent big enzyme molecules from leaving while allowing small substrates and products to pass through to maintain the enzyme activity. The permeability properties of Alg-g-PEG/α-CD hollow nanospheres were investigated by encapsulating different probes. The results showed that the molar mass cutoff (MMCO) of these hollow nanospheres was between 20 and 40 kDa. The encapsulation capability of Alg-g-PEG/α-CD hollow nanospheres was also investigated, the results indicated a maximal values for L-asparaginase encapsulation. Furthermore, the encapsulation behavior for L-asparaginase showed PEG graft density (GD) dependence.