Microbial delignification of corn stover by Ceriporiopsis subvermispora for improving cellulose digestibility
Microbial delignification of corn stover by Ceriporiopsis subvermispora for improving cellulose digestibility
复制标题
DOI：
10.1016/j.enzmictec.2010.04.001
复制
发表时间：
2010-07-05
影响因子：
3.4
通讯作者：
Li, Yebo
中科院分区：
文献类型：
--
作者：
Wan, Caixia;Li, Yebo
评估了固态培养中白腐真菌 Ceriporiopsis subvermispora 对玉米秸秆的脱木质素，以改善随后的酶水解。结果表明，在42 d的培养过程中，C. subvermispora选择性降解木质素高达39.2%，而纤维素损失小于5%。然而，随着木质素的降解，半纤维素损失高达 27.0%。在 C. subvermispora 降解玉米秸秆的过程中检测到木质素分解酶、MnP 和漆酶。对于主要水解酶，木聚糖酶是唯一检测到的酶。经C. subvermispora脱木素的玉米秸秆酶解产率显着提高，预处理18-42 d后，总葡萄糖产率达到约57-67%，而未经处理的葡萄糖产率仅为22%。当用辅助木聚糖酶/纤维素酶复合物补充经过 18 天处理的玉米秸秆的酶水解时，获得了约 72% 的较高总葡萄糖产率。 (C) 2010 Elsevier Inc. 保留所有权利。
Delignification of corn stover by the white rot fungus Ceriporiopsis subvermispora in solid-state cultivation was evaluated for improving subsequent enzymatic hydrolysis. The results showed that C. subvermispora selectively degraded lignin by as much as 39.2% while the cellulose loss was less than 5% during 42 d of cultivation. However, hemicellulose loss of up to 27.0% was concomitant with the lignin degradation. Ligninolytic enzymes, MnP and laccase, were detected during degradation of corn stover by C. subvermispora. For major hydrolytic enzymes, xylanase was the only enzyme detected. The enzymatic hydrolysis yield of corn stover delignified by C. subvermispora was remarkably improved, reaching about 57-67% overall glucose yield after 18-42 d of pretreatment, while the glucose yield of the untreated was only 22%. A higher overall glucose yield of about 72% was obtained when the enzymatic hydrolysis of the 18 d treated corn stover was supplemented with an accessory xylanase/cellulase enzyme complex. (C) 2010 Elsevier Inc. All rights reserved.