Effect of electrical polarization of hydroxyapatite ceramics on new bone formation
Effect of electrical polarization of hydroxyapatite ceramics on new bone formation
复制标题
DOI：
10.1007/s00223-005-0213-6
复制
发表时间：
2006-03-01
影响因子：
4.2
通讯作者：
Itoh, S
中科院分区：
文献类型：
--
作者：
Itoh, S;Nakamura, S;Itoh, S
通过质子传输极化可以在羟基磷灰石（HAp）陶瓷上感应出大的表面电荷，但这不会影响β-磷酸三钙（TCP），因为其极化率低。我们希望使用颅骨骨缺损模型来检查表面带正电或负电的 HAp 和 HAp/TCP 板之间成骨细胞活性和新骨生长的差异。在第一组大鼠中，将测试片带正电的表面朝下放置在硬脑膜上。在第二组中，将测试件带负电的表面朝下放置在硬脑膜上。第三组接受不带电的测试件。进行组织学检查，包括成骨细胞和破骨细胞的酶染色。虽然在颅骨膜处没有观察到骨形成，但颅骨碎片上的直接骨形成和新骨生长从损伤部位的边缘扩展，跨越HAp和HAp/TCP板的带正电和负电的表面。植入板的电极化（包括正电荷）导致成骨细胞活性增强，但在带正电荷的板表面上观察到破骨细胞活性降低。因此，HAp 陶瓷的极化可以调节新骨的形成和吸收。
Large surface charges can be induced on hydroxyapatite (HAp) ceramics by proton transport polarization, but this does not affect beta-tricalcium phosphate (TCP) because of its low polarizability. We wished to examine differences in osteogenic cell activity and new bone growth between positively or negatively surface-charged HAp and HAp/TCP plates using a calvarial bone defect model. In the first group of rats, test pieces were placed with their positively charged surfaces face down on the dura mater. In the second group, test pieces were placed with their negatively charged surfaces face down on the dura mater. A third group received noncharged test pieces. Histological examination, including enzymatic staining for osteoblasts and osteoclasts, was carried out. While no bone formation was observed at the pericranium, direct bone formation on the cranial bone debris and new bone growth expanded from the margins of the sites of injury to bridge across both the positively and negatively charged surfaces of HAp and HAp/TCP plates occurred. Electrical polarization of implanted plates, including positive charge, led to enhanced osteoblast activity, though decreased osteoclast activity was seen on the positively charged plate surface. Thus, polarization of HAp ceramics may modulate new bone formation and resorption.