Contribution of the FeoB transporter to Streptococcus suis virulence
Contribution of the FeoB transporter to Streptococcus suis virulence
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DOI：
10.2436/20.1501.01.91
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发表时间：
2009-06-01
影响因子：
3.1
通讯作者：
Barbe, Jordi
中科院分区：
文献类型：
--
作者：
Aranda, Jesus;Cortes, Pilar;Barbe, Jordi
尽管铁转运系统是几种革兰氏阴性细菌的全部毒力所必需的，但铁转运系统对链球菌致病特性的贡献在很大程度上是未知的。在这项工作中，我们表明，猪病原体和人畜共患病剂猪链球菌有一个feo AB操纵子类似的编码所有铁转运系统在大肠杆菌。电泳迁移率分析和转录分析证实，S。猪Feo基因受铁摄取调节蛋白（Fur）的负调控。使用feoB缺陷突变株进行小鼠体内试验，以研究该基因对S.猪。结果表明，突变株的半数致死量（LD 50）比野生型亲本株高约10倍。这些数据表明Feo金属转运蛋白在链球菌感染性疾病中起重要作用。这与之前报道的其他革兰氏阳性细菌中相同基因的结果相反。[Int微生物学2009; 12（2）：137-141]
The contribution of iron transporter systems encoded by feo genes to the pathogenic traits of streptococci is largely unknown, despite the fact that those systems are required for the full virulence of several gram-negative bacterial species. In this work, we show that the swine pathogen and zoonotic agent Streptococcus suis has a feo AB operon similar to that encoding all iron transporter system in Escherichia coli. Electrophoretic mobility assays and transcriptional analyses confirmed that the expression of S. suis feo genes is under the negative control of the ferric uptake regulator (Fur) protein. In vivo trials in mice using a feoB defective Mutant strain were carried out to investigate the contribution of this gene to the virulence of S. suis. The results showed that the median lethal dose (LD50) of the Mutant was approximately 10-fold higher than that of the wild-type parent strain. These data Suggest that the Feo metal transporter plays a significant role in streptococcal infectious disease. This is in contrast to previous results reported for this same gene in other gram-positive bacterial species. [Int Microbiol 2009; 12(2):137-141]