Expression of the testis-specific histone H1t gene: evidence for involvement of multiple cis-acting promoter elements.
Expression of the testis-specific histone H1t gene: evidence for involvement of multiple cis-acting promoter elements.
复制标题
睾丸特异性组蛋白 H1t 基因的表达：多个顺式作用启动子元件参与的证据。
DOI：
10.1021/bi00038a045
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Grimes,SR
中科院分区：
文献类型：
--
作者：
Wolfe,SA;vanWert,JM;Grimes,SR
修订稿于 1995 年 7 月 14 日收到®摘要：组蛋白 Hit 基因仅在睾丸初级精母细胞中表达。先前的研究表明，Hit mRNA 的积累仅发生在正常大鼠和携带大鼠 Hit 转基因的转基因小鼠的初级精母细胞中。在这项研究中，比较了人、猴、小鼠和大鼠Hit基因的DNA序列，发现从H1/AC盒延伸到TATAA盒的近端启动子区域几乎相同。除了复制依赖性 HI 启动子共有的保守元件外，Hit 启动子还包含一个独特的 TE 元件，该元件内的序列可能有助于增强原代精母细胞中基因的表达。两个不完美的反向重复序列指定为 TE1 和 TE2，位于较大的 TE 元件内，与富含 GC 的中央区域重叠，并与源自初级精母细胞的核蛋白特异性结合。以甲基化干扰和紫外交联实验为特征的蛋白质相互作用表明，分子量约为 180 kDa 的蛋白质复合物与 TE1 结合。 Hit 和一些复制依赖性组蛋白 HI 启动子中的富含 GC 的区域包含 Spl 共有序列。尽管含有富含GC区域的Hlt/TE元件结合核蛋白，但它似乎不结合从富含原代精母细胞的细胞群获得的Spl，如使用多克隆抗Spl抗体的电泳迁移率超移测定所确定的。
Revised Manuscript Received July 14, 1995® abstract: The histone Hit gene is expressed exclusively in testis primary spermatocytes. Previous studies indicate that accumulation of Hit mRNA occurs only in primary spermatocytes in normal rats and in transgenic mice bearing the rat Hit transgene. In this study, DNA sequences of human, monkey, mouse, and rat Hit genes were compared and found to be almost identical in the proximal promoter region extending from the Hl/AC box through the TATAA box. In addition to conserved elements common to replication-dependent HI promoters, the Hit promoter contains a unique TE element, andsequences within this element may contribute to enhancedexpression of the gene in primary spermatocytes. Two imperfect inverted repeat sequences designated TE1 and TE2, that are located within the larger TE element, overlap a central GC-rich region and bind specifically to nuclear proteins derived from primary spermatocytes. Protein interactions characterized by methylation interference and UV cross-linking experiments indicate that a complex of proteins with a molecular mass of approximately 180 kDa binds TE1. The GC-rich region in Hit and in some replication dependent histone HI promoters contains an Spl consensus sequence. Although the Hlt/TE element that contains the GC-rich region binds nuclear proteins, it does not appear to bind Spl obtained from cell populations enriched in primary spermatocytes as determined by electrophoretic mobility supershift assays using polyclonalanti-Spl antibodies.